Molecular interaction and cellular location of RecA and CheW proteins in Salmonella enterica during SOS response and their implication in swarming
Irazoki, Oihane (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Aranda Rodríguez, Jesús (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Zimmermann, Timo (Centre de Regulació Genòmica)
Campoy Sánchez, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Barbé García, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2016
Abstract:	In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed.
Grants:	Ministerio de Economía y Competitividad BIO2016-77011-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-572
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	SOS response ; Swarming ; Chemoreceptor polar arrays ; Chemosensory cluster assembly ; RecA ; CheW ; 3D-STED
Published in:	Frontiers in microbiology, Vol. 7 (October 2016) , art. 1560, ISSN 1664-302X

DOI: 10.3389/fmicb.2016.01560
PMID: 27766091

15 p, 5.3 MB

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