Revisiting the taxonomical classification of Porcine Circovirus type 2 (PCV2) : still a real challenge
Franzo, Giovanni (University of Padua. Department of Animal Medicine, Production and Health)
Cortey, Martí (The Pirbright Institute (Regne Unit))
Olvera, Alex (IrsiCaixa - HIVACAT)
Novosel, Dinko (Croatian Veterinary Institute. Department of Pathology)
De Castro, Alessandra Marnie Martins Gomes (University of Sao Paulo. Department of Preventive Veterinary Medicine and Animal Health)
Biagini, Philippe (Unité de Virologie Moléculaire, Emergence et Co-évolution Virale)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Drigo, Michele (University of Padua. Department of Animal Medicine, Production and Health)

Fecha:	2015
Resumen:	PCV2 has emerged as one of the most devastating viral infections of swine farming, causing a relevant economic impact due to direct losses and control strategies expenses. Epidemiological and experimental studies have evidenced that genetic diversity is potentially affecting the virulence of PVC2. The growing number of PCV2 complete genomes and partial sequences available at GenBank questioned the accepted PCV2 classification. Nine hundred seventy five PCV2 complete genomes and 1,270 ORF2 sequences available from GenBank were subjected to recombination, PASC and phylogenetic analyses and results were used for comparison with previous classification scheme. The outcome of these analyses favors the recognition of four genotypes on the basis of ORF2 sequences, namely PCV2a, PCV2b, PCV2c and PCV2d-mPCV2b. To deal with the difficulty of founding an unambiguous classification and accounting the impossibility to define a p-distance cut-off, a set of reference sequences that could be used in further phylogenetic studies for PCV2 genotyping was established. Being aware that extensive phylogenetic analyses are time-consuming and often impracticable during routine diagnostic activity, ORF2 nucleotide positions adequately conserved in the reference sequences were identified and reported to allow a quick genotype differentiation. Globally, the present work provides an updated scenario of PCV2 genotypes distribution and, based on the limits of the previous classification criteria, proposes new rapid and effective schemes for differentiating the four defined PCV2 genotypes. The online version of this article (doi:10. 1186/s12985-015-0361-x) contains supplementary material, which is available to authorized users.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Porcine circovirus
Publicado en:	Virology Journal, Vol. 12 (august 2015) , ISSN 1743-422X

DOI: 10.1186/s12985-015-0361-x
PMID: 26311322

8 p, 959.2 KB

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