Zebrafish Models for Human Acute Organophosphorus Poisoning
Faria, Melissa (IDÆA-CSIC)
Garcia-Reyero, Natàlia (Mississippi State University. Institute for Genomics, Biocomputing & Biotechnology)
Padrós, Francesc 1965- (Universitat Autònoma de Barcelona. Servei de Diagnòstic Patològic en Peixos)
Babin, Patrick J. (Université de Bordeaux. Rare Diseases, Genetic and Metabolism)
Sebastián, David (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Cachot, Jérôme (Université de Bordeaux)
Prats, Eva (CID-CSIC)
Arick II, Mark (US Army Engineer Research and Development Center. Environmental Laboratory)
Rial, Eduardo (Department of Cellular and Molecular Medicine)
Knoll-Gellida, Anja (Université de Bordeaux. Rare Diseases, Genetic and Metabolism)
Mathieu, Guilaine (Université de Bordeaux. Rare Diseases, Genetic and Metabolism)
Le Bihanic, Florane (Université de Bordeaux)
Escalon, B. Lynn (Université de Bordeaux)
Zorzano Olarte, Antonio (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Soares, Amadeu M. V. M. (University of Aveiro. Department of Biology)
Raldúa, Demetrio (IDÆA-CSIC)

Date:	2015
Abstract:	Terrorist use of organophosphorus-based nerve agents and toxic industrial chemicals against civilian populations constitutes a real threat, as demonstrated by the terrorist attacks in Japan in the 1990 s or, even more recently, in the Syrian civil war. Thus, development of more effective countermeasures against acute organophosphorus poisoning is urgently needed. Here, we have generated and validated zebrafish models for mild, moderate and severe acute organophosphorus poisoning by exposing zebrafish larvae to different concentrations of the prototypic organophosphorus compound chlorpyrifos-oxon. Our results show that zebrafish models mimic most of the pathophysiological mechanisms behind this toxidrome in humans, including acetylcholinesterase inhibition, N-methyl-D-aspartate receptor activation, and calcium dysregulation as well as inflammatory and immune responses. The suitability of the zebrafish larvae to in vivo high-throughput screenings of small molecule libraries makes these models a valuable tool for identifying new drugs for multifunctional drug therapy against acute organophosphorus poisoning.
Grants:	Ministerio de Economía y Competitividad CTM2014-51985-R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 5 (october 2015) , ISSN 2045-2322

DOI: 10.1038/srep15591
PMID: 26489395

15 p, 1.9 MB

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