Long-term seasonal and interannual variability of marine aerobic anoxygenic photoheterotrophic bacteria
Auladell, Adrià (Institut de Ciències del Mar)
Sanchez, Pablo (Institut de Ciències del Mar)
Sánchez Martínez, M. Olga (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia.)
Gasol, Josep M. (Institut de Ciències del Mar)
Ferrera, Isabel (Institut de Ciències del Mar)

Fecha:	2019
Descripción:	13 pàg.
Resumen:	We studied the long-term temporal dynamics of the aerobic anoxygenic phototrophic (AAP) bacteria, a relevant functional group in the coastal marine microbial food web, using high-throughput sequencing of the pufM gene coupled with multivariate, time series and co-occurrence analyses at the Blanes Bay Microbial Observatory (NW Mediterranean). Additionally, using metagenomics, we tested whether the used primers captured accurately the seasonality of the most relevant AAP groups. Phylogroup K (Gammaproteobacteria) was the greatest contributor to community structure over all seasons, with phylogroups E and G (Alphaproteobacteria) being prevalent in spring. Diversity indices showed a clear seasonal trend, with maximum values in winter, which was inverse to that of AAP abundance. Multivariate analyses revealed sample clustering by season, with a relevant proportion of the variance explained by day length, temperature, salinity, phototrophic nanoflagellate abundance, chlorophyll a, and silicate concentration. Time series analysis showed robust rhythmic patterns of co-occurrence, but distinct seasonal behaviors within the same phylogroup, and even within different amplicon sequence variants (ASVs) conforming the same operational taxonomic unit (OTU). Altogether, our results picture the AAP assemblage as highly seasonal and recurrent but containing ecotypes showing distinctive temporal niche partitioning, rather than being a cohesive functional group.
Ayudas:	Ministerio de Economía y Competitividad CTM2015-70340-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1568
Nota:	Altres ajuts: access to the MareNostrum Supercomputer was granted to Ramiro Logares under agreement BCV-2017-3-0001.
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Environmental sciences ; Microbial ecology
Publicado en:	The ISME Journal, Vol. 13 (2019) , p. 1975-1987, ISSN 1751-7370

DOI: 10.1038/s41396-019-0401-4
PMID: 30914777
PMID: 30914777

Postprint 34 p, 1.1 MB

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