Micrornas as a suitable biomarker to detect the effects of long-term exposures to nanomaterials. Studies on tio2np and mwcnt
Ballesteros Ribera, Sandra (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Vales, Gerard (Finnish Institute of Occupational Health)
Velázquez Henar, Antonia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Pastor Benito, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Alaraby, Mohamed (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2021
Abstract:	The presence of nanomaterials (NMs) in the environment may represent a serious risk to human health, especially in a scenario of chronic exposure. To evaluate the potential relationship between NM-induced epigenetic alterations and carcinogenesis, the present study analyzed a panel of 33 miRNAs related to the cell transformation process in BEAS-2B cells transformed by TiO2NP and long-term MWCNT exposure. Our battery revealed a large impact on miRNA expression profiling in cells exposed to both NMs. From this analysis, a small set of five miRNAs (miR-23a, miR-25, miR-96, miR-210, and miR-502) were identified as informative biomarkers of the transforming effects induced by NM exposures. The usefulness of this reduced miRNA battery was further vali-dated in other previously generated transformed cell systems by long-term exposure to other NMs (CoNP, ZnONP, MSiNP, and CeO2NP). Interestingly, the five selected miRNAs were consistently overexpressed in all cell lines and NMs tested. These results confirm the suitability of the proposed set of mRNAs to identify the potential transforming ability of NMs. Particular attention should be paid to the epigenome and especially to miRNAs for hazard assessment of NMs, as wells as for the study of the underlying mechanisms of action.
Grants:	Agencia Estatal de Investigación PID2020-116789RB-C43
Note:	Altres ajuts: Universitat Autònoma de Barcelona PIF-UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	MicroRNAs ; Oncogenesis ; Carcinogenesis ; Cell transformation ; Nanomaterials ; Long-term exposures ; TiO2NP ; MWCNT
Published in:	Nanomaterials, Vol. 11, Issue 12 (December 2021) , art. 3458, ISSN 2079-4991

DOI: 10.3390/nano11123458
PMID: 34947804

14 p, 3.2 MB

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