Addition of reduced glutathione (GSH) to freezing medium reduces intracellular ROS levels in donkey sperm
Yánez-Ortiz, Iván (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Catalán, Jaime (Universitat de Girona. Departament de Biologia)
Delgado Bermudez, Ariadna (Universitat de Girona. Departament de Biologia)
Carluccio, Augusto (University of Teramo. Faculty of Veterinary Medicine)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)

Date:	2021
Abstract:	In donkeys, the use of frozen-thawed sperm for artificial insemination (AI) leads to low fertility rates. Furthermore, donkey sperm produce a large amount of reactive oxygen species (ROS), and post-AI inflammation induces the formation of neutrophil extracellular traps (NETosis), which further generates many more ROS. These high ROS levels may induce lipid peroxidation in the sperm plasma membrane, thus affecting its integrity. Enzymatic and non-enzymatic antioxidants, mainly found in the seminal plasma (SP), are responsible for maintaining the redox balance. However, this fluid is removed prior to cryopreservation, thereby exposing sperm cells to further oxidative stress. The exogenous addition of antioxidants to the freezing medium can reduce the detrimental effects caused by ROS generation. Therefore, the aim of this study was to evaluate how the addition of different reduced glutathione (GSH) concentrations (control, 2 mM, 4 mM, 6 mM, 8 mM, and 10 mM) to fresh sperm affect their cryotolerance. Total and progressive motility, kinematic parameters and motile sperm subpopulations were significantly (p < 0. 05) different from the control in treatments containing 8 mM and 10 mM GSH, but not at lower concentrations. Plasma and acrosome membrane integrity, mitochondrial membrane potential (MMP) and intracellular superoxide levels (O ) were not affected (p.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1229 Agencia Estatal de Investigación AGL2017-88329-R
Note:	Funding: GENCAT (2017-SGR-1229).
Note:	Altres ajuts: SENESCYT/CZ02-000507-2019
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Sperm cryopreservation ; Reactive oxygen species (ROS) ; Superoxide anion (O2-) ; Reduced glutathione (GSH) ; Antioxidants ; Donkey
Published in:	Veterinary sciences, Vol. 8 Núm. 12 (december 2021) , p. 302, ISSN 2306-7381

DOI: 10.3390/vetsci8120302
PMID: 34941829

17 p, 1.8 MB

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