Prevalence of heterotrophic methylmercury detoxifying bacteria across oceanic regions
Sanz-Sáez, Isabel (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)
Pereira Garcia, Carla (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)
Bravo, Andrea G. (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)
Trujillo, Laura (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)
Pla i Ferriol, Martí (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Miguel, Capilla (Universitat de València. Departament d'Enginyeria Química)
Sánchez, Pablo (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)
Rodríguez Martín-Doimeadios, Rosa Carmen (Universidad de Castilla-La Mancha. Departamento de Química Analítica y Tecnología de Alimentos)
Acinas, Silvia G. (Institut de Ciències del Mar. Departament de Biologia Marina i Oceanografia)
Sánchez Martínez, M. Olga (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Fecha:	2022
Resumen:	Microbial reduction of inorganic divalent mercury (Hg2+) and methylmercury (MeHg) demethylation is performed by the mer operon, specifically by merA and merB genes, respectively, but little is known about the mercury tolerance capacity of marine microorganisms and its prevalence in the ocean. Here, combining culture-dependent analyses with metagenomic and metatranscriptomic data, we show that marine bacteria that encode mer genes are widespread and active in the global ocean. We explored the distribution of these genes in 290 marine heterotrophic bacteria (Alteromonas and Marinobacter spp. ) isolated from different oceanographic regions and depths, and assessed their tolerance to diverse concentrations of Hg2+ and MeHg. In particular, the Alteromonas sp. ISS312 strain presented the highest tolerance capacity and a degradation efficiency for MeHg of 98. 2% in 24 h. Fragment recruitment analyses of Alteromonas sp. genomes (ISS312 strain and its associated reconstructed metagenome assembled genome MAG-0289) against microbial bathypelagic metagenomes confirm their prevalence in the deep ocean. Moreover, we retrieved 54 merA and 6 merB genes variants related to the Alteromonas sp. ISS312 strain from global metagenomes and metatranscriptomes from Tara Oceans. Our findings highlight the biological reductive MeHg degradation as a relevant pathway of the ocean Hg biogeochemical cycle.
Ayudas:	European Commission 749645 Ministerio de Educación, Cultura y Deporte FPU14/03590
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Mercury ; Methylmercury ; Marine bacteria ; Mercury-resistant bacteria ; MerA ; MerB ; Minimum inhibitory concentration (MIC)
Publicado en:	Environmental Science & Technology, Vol. 56, Num. 6 (March 2022) , p. 3452-3461, ISSN 1520-5851

DOI: 10.1021/acs.est.1c05635
PMID: 35245029

Postprint 24 p, 20.9 MB

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