Changes in Oxidative Status Biomarkers in Saliva and Serum in the Equine Gastric Ulcer Syndrome and Colic of Intestinal Aetiology : a Pilot Study
Contreras-Aguilar, M. D. (Universidad de Murcia. Departamento de Medicina y Cirugía Animal)
Rubio, Camila Peres (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
González-Aróstegui, Luis Guillermo (Universidad de Murcia. Departamento de Medicina y Cirugía Animal)
Martín-Cuervo, María (Universidad de Extremadura. Departamento de Medicina Animal)
Cerón, José Joaquín (Universidad de Murcia. Departamento de Medicina y Cirugía Animal)
Ayala, Ignacio (Universidad de Murcia. Departamento de Medicina y Cirugía Animal)
Henriksen, Ida Marie (University of Copenhagen. Department of Veterinary Clinical Sciences)
Jacobsen, Stine (University of Copenhagen. Department of Veterinary Clinical Sciences)
Hansen, Sanni (University of Copenhagen. Department of Veterinary Clinical Sciences)

Date:	2022
Abstract:	Changes in the oxidative status of the blood of horses suffering from gastric ulcers and colic of intestinal aetiology (CIE) have been reported. However, saliva can also be a source of biomarkers of oxidative status. Therefore, this study aims to validate automated assays for the measurement of oxidative status biomarkers (ferric reducing ability of saliva/serum-FRAS/FRAP, cupric reducing antioxidant capacity-CUPRAC, the Trolox equivalent antioxidant capacity-TEAC, uric acid, and advanced oxidation protein products-AOPP) in the saliva and serum of horses, to assess their changes in the different ulcer gastric diseases (squamous-ESGD and glandular-EGGD) and CIE, and to evaluate their relationship with serum amyloid A (SAA), adenosine deaminase (ADA), and the systemic inflammatory response syndrome (SIRS) status. The assays showed a low imprecision and good linearity with enough sensitivity in both fluids. In EGGD, higher levels of FRAS, uric acid, and AOPP in saliva were observed compared to the healthy group, correlating with the salivary ADA levels. Horses with CIE showed increases in uric acid concentrations in serum associated with their SIRS status and outcome of the disease. In conclusion, analytes related to the oxidative status can be measured in the saliva and serum from horses by automated assays, and some of them can potentially be assessed as biomarkers in horses with gastric ulcers and CIE.
Grants:	Ministerio de Economía y Competitividad FJC2019-042475-I
Note:	Altres ajuts: Gobierno Regional Murcia, programa Séneca 19894/GERM/15
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Horse ; EGUS ; Colic ; Redox profile ; Saliva
Published in:	Animals, Vol. 12 Núm. 5 (3-1 2022) , p. 667, ISSN 2076-2615

DOI: 10.3390/ani12050667
PMID: 35268236

14 p, 1.3 MB

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