A plug, print & play inkjet printing and impedance-based biosensing technology operating through a smartphone for clinical diagnostics
Rosati, Giulio (Institut Català de Nanociència i Nanotecnologia)
Urban, Massimo (University of Trieste. Department of Chemical and Pharmaceutical Sciences)
Zhao, Lei (Institut Català de Nanociència i Nanotecnologia)
Yang, Qiuyue (Institut Català de Nanociència i Nanotecnologia)
De Carvalho Castro Silva, Cecilia (Institut Català de Nanociència i Nanotecnologia)
Bonaldo, Stefano (University of Padova. Department of Information Engineering)
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Nguyen, Emily P. (Institut Català de Nanociència i Nanotecnologia)
Ortega, Gabriel (University of California Santa Barbara. Department of Chemistry and Biochemistry)
Fornasiero, Paolo (University of Trieste. Department of Chemical and Pharmaceutical Sciences)
Paccagnella, Alessandro (University of Padova. Department of Information Engineering)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Fecha:	2022
Resumen:	Simplicity is one of the key feature for the spread of any successful technological product. Here, a method for rapid and low-cost fabrication of electrochemical biosensors is presented. This "plug, print & play" method involves inkjet-printing even in an office-like environment, without the need of highly specialized expertise or equipment, guaranteeing an ultra-fast idea to (scaled) prototype production time. The printed biosensors can be connected to a smartphone through its audio input for their impedance readout, demonstrating the validity of the system for point-of-care biosensing. Proper electrodes layout guarantees high sensitivity and is validated by finite element simulations. The introduction of a passivation method (wax printing) allowed to complete the devices fabrication process, increasing their sensitivity. Indeed, the wax allowed reducing the interference related to the parasitic currents flowing through the permeable coating of the employed substrates, which was used for the chemical sintering, thus avoiding the common thermal treatment after printing. As a case study, we used the devices to develop an electrochemical aptamer-based sensor for the rapid detection of neutrophil gelatinase-associated lipocalin (NGAL) in urine - a clinically important marker of acute kidney injury. The aptasensor platform is capable of detecting clinically relevant concentrations of NGAL with a simple and rapid smartphone readout. The developed technology may be extended in the future to continuous monitoring, taking advantage of its flexibility to integrate it in tubes, or to other diagnostic applications where cost/efficiency and rapidity of the research, development and implementation of point of care devices is a must.
Ayudas:	European Commission 825694 European Commission 754510 European Commission 101008701
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Nanofunctional inks ; Inkjet-printed sensors ; Aptasensors ; Flexible electronics ; Impedimetric biosensors ; Smartphone readout
Publicado en:	Biosensors & bioelectronics, Vol. 196 (January 2022) , art. 113737, ISSN 1873-4235

DOI: 10.1016/j.bios.2021.113737

10 p, 4.6 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
Artículos > Artículos de investigación
Artículos > Artículos publicados