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Página principal > Artículos > Artículos publicados > Synchrotron-based fourier-transform infrared micro-spectroscopy (SR-FTIRM) fingerprint of the small anionic molecule cobaltabis (dicarbollide) uptake in glioma stem cells |
Fecha: | 2021 |
Resumen: | The anionic cobaltabis (dicarbollide) [3,3'-Co(1,2-CBH)] −, [ o -COSAN] −, is the most studied icosahedral metallacarborane. The sodium salts of [ o -COSAN] − could be an ideal candidate for the anti-cancer treatment Boron Neutron Capture Therapy (BNCT) as it possesses the ability to readily cross biological membranes thereby producing cell cycle arrest in cancer cells. BNCT is a cancer therapy based on the potential of 10 B atoms to produce α particles that cross tissues in which the 10 B is accumulated without damaging the surrounding healthy tissues, after being irradiated with low energy thermal neutrons. Since Na[ o -COSAN] displays a strong and characteristic ν(B-H) frequency in the infrared range 2. 600-2. 500 cm −1, we studied the uptake of Na[ o -COSAN] followed by its interaction with biomolecules and its cellular biodistribution in two different glioma initiating cells (GICs), mesenchymal and proneural respectively, by using Synchrotron Radiation-Fourier Transform Infrared (FTIR) micro-spectroscopy (SR-FTIRM) facilities at the MIRAS Beamline of ALBA synchrotron light source. The spectroscopic data analysis from the bands in the regions of DNA, proteins, and lipids permitted to suggest that after its cellular uptake, Na[ o -COSAN] strongly interacts with DNA strings, modifies proteins secondary structure and also leads to lipid saturation. The mapping suggests the nuclear localization of [ o -COSAN] −, which according to reported Monte Carlo simulations may result in a more efficient cell-killing effect compared to that in a uniform distribution within the entire cell. In conclusion, we show pieces of evidence that at low doses, [ o -COSAN] − translocates GIC cells' membranes and it alters the physiology of the cells, suggesting that Na[ o -COSAN] is a promising agent to BNCT for glioblastoma cells. |
Ayudas: | Ministerio de Sanidad y Consumo FIS-PI18/00916 Agencia Estatal de Investigación PID2019-106832RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1720 |
Nota: | Altres ajuts: CELLS-ALBA Synchroton (Ref.: 2019023533) |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió publicada |
Materia: | Boron neutron capture therapy ; Cobaltabis (dicarbollide) ; DNA interactions ; Glioblastoma ; Glioma initiating cells ; Inorganic small molecules ; Lipid saturation ; SR-FTIRM |
Publicado en: | International journal of molecular sciences, Vol. 22, Issue 18 (September 2021) , art. 9937, ISSN 1422-0067 |
25 p, 6.6 MB |