A genome-wide association study identifies novel players in Na and Fe homeostasis in Arabidopsis thaliana under alkaline-salinity stress
Almira, Maria Jose (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)
Pérez-Martín, Laura (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)
Busoms, Silvia (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)
Boesten, René (Wageningen University and Research. Laboratory of Genetics)
Llugany i Ollé, Mercè (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)
Aarts, G. Mark (Wageningen University and Research. Laboratory of Genetics)
Poschenrieder, Charlotte (Universitat Autònoma de Barcelona. Laboratori de Fisiologia Vegetal)

Date:	2023
Abstract:	In nature, multiple stress factors occur simultaneously. The screening of natural diversity panels and subsequent Genome-Wide Association Studies (GWAS) is a powerful approach to identify genetic components of various stress responses. Here, the nutritional status variation of a set of 270 natural accessions of Arabidopsis thaliana grown on a natural saline-carbonated soil is evaluated. We report significant natural variation on leaf Na (LNa) and Fe (LFe) concentrations in the studied accessions. Allelic variation in the NINJA and YUC8 genes is associated with LNa diversity, and variation in the ALA3 is associated with LFe diversity. The allelic variation detected in these three genes leads to changes in their mRNA expression and correlates with plant differential growth performance when plants are exposed to alkaline salinity treatment under hydroponic conditions. We propose that YUC8 and NINJA expression patters regulate auxin and jasmonic signaling pathways affecting plant tolerance to alkaline salinity. Finally, we describe an impairment in growth and leaf Fe acquisition associated with differences in root expression of ALA3, encoding a phospholipid translocase active in plasma membrane and the trans Golgi network which directly interacts with proteins essential for the trafficking of PIN auxin transporters, reinforcing the role of phytohormonal processes in regulating ion homeostasis under alkaline salinity.
Grants:	Agencia Estatal de Investigación PID2019-104000-RB-I00
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Alkaline-salinity ; GWAS ; Arabidopsis ; Natural variation ; Ionome
Published in:	The Plant journal, Vol. 113, Issue 2 (January 2023) , p. 225-245, ISSN 1365-313X

DOI: 10.1111/tpj.16042
PMID: 36433704

21 p, 1.3 MB

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