Comparative Genomics of Clinical and Environmental Isolates of Vibrio spp. of Colombia : Implications of Traits Associated with Virulence and Resistance
Pérez-Duque, Alejandra (Centro de Bioinformática y Biología Computacional BIOS)
Gonzalez-Muñoz, Andrea (Centro de Bioinformática y Biología Computacional BIOS)
Arboleda-Valencia, Jorge (Centro de Bioinformática y Biología Computacional BIOS)
Vivas-Aguas, Lizbeth Janet (Instituto de Investigaciones Marinas y Costeras José Benito Vives De Andreis INVEMAR)
Córdoba-Meza, Tania (Instituto de Investigaciones Marinas y Costeras José Benito Vives De Andreis INVEMAR)
Rodriguez-Rey, Ghennie Tatiana (Universidad de Caldas. BIONAT: Grupo de Investigaciones en Biodiversidad y Recursos Naturales)
Díaz-Guevara, Paula (Instituto Nacional de Salud. Grupo de Microbiología (Bogotá))
Martinez-Urtaza, Jaime (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Wiesner-Reyes, Magdalena (Instituto Nacional de Salud. Grupo de Microbiología (Bogotá))

Date:	2021
Abstract:	There is widespread concern about the increase in cases of human and animal infections caused by pathogenic Vibrio species due to the emergence of epidemic lineages. In Colombia, active surveillance by the National Institute of Health (INS) has confirmed the presence of Vibrio; however, in routine surveillance, these isolates are not genomically characterized. This study focused on the pangenome analysis of six Vibrio species: V. parahaemolyticus, V. vulnificus, V. alginolyticus, V. fluvialis, V. diabolicus and V. furnissii to determine the genetic architectures of potentially virulent and antimicrobial resistance traits. Isolates from environmental and clinical samples were genome sequenced, assembled and annotated. The most important species in public health were further characterized by multilocus sequence typing and phylogenomics. For V. parahaemolyticus, we found the virulent ST3 and ST120 genotypes. For V. vulnificus, we identified isolates belonging to lineages 1 and 2. Virulence gene homologues between species were found even in non-pathogenic species such as V. diabolicus. Annotations related to the mobilome, integrative mobile and conjugative elements and resistance genes were obtained from environmental and clinical isolates. This study contributes genomic information to the intensified surveillance program implemented by the INS to establish potential sources of vibriosis in Colombia.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Vibriosis ; Virulence ; Antibiotic resistance ; Pangenome ; Whole genome sequencing ; SDG 3 - Good Health and Well-being
Published in:	Pathogens, Vol. 10, Num. 12 (December 2021) , art. 1605, ISSN 2076-0817

DOI: 10.3390/pathogens10121605
PMID: 34959560

22 p, 5.2 MB

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