Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats
Subias-Gusils, Alex (Universitat Autònoma de Barcelona. Departament de Psiquiatria i de Medicina Legal)
Álvarez-Monell, Adam (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Boqué, Noemí (Eurecat, Centre Tecnològic de Catalunya, Biotechnology Area and Technological Unit of Nutrition and Health)
Caimari, Antoni (Eurecat, Centre Tecnològic de Catalunya, Biotechnology Area and Technological Unit of Nutrition and Health)
Mariné-Casadó, Roger (Eurecat, Centre Tecnològic de Catalunya, Biotechnology Area and Technological Unit of Nutrition and Health)
Escorihuela, Rosa M (Universitat Autònoma de Barcelona. Departament de Psiquiatria i de Medicina Legal)
Solanas i Garcia, Montserrat (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date:	2023
Abstract:	Supplementation with natural bioactive compounds has been proposed to be a complementary tool to the calorie-restricted diets and physical exercise programs used to tackle human overweight, obesity and Metabolic syndrome. Herein, we evaluated the effects of 14 weeks of calorie-restricted cafeteria diet either alone or combined with oral administration of the polyphenol oleuropein in obese adult male rats, compared with a control group fed standard chow and a group fed cafeteria diet. Animals were sacrificed at the age of 26 weeks and several tissues of interest were removed. The results showed that both dietary interventions reduced the adiposity index (p < 0. 05 and p < 0. 01, respectively), and specifically the abdominal fat depots (mesenteric: p < 0. 01 and p < 0. 01, respectively; and epididymal: both diets p < 0. 001) and restored the decreased soleus skeletal muscle mass. Both interventions decreased leptin mRNA expression in mesenteric white adipose tissue (p < 0. 05) and normalized hypothalamic Agrp mRNA expression compared to cafeteria-fed obese rats (p < 0. 05). However, only the calorie-restricted cafeteria diet supplemented with oleuropein induced additional lower retroperitoneal adipose accretion (p < 0. 05) and increased hypothalamic leptin receptor mRNA levels (p < 0. 05). Experiments with female animals, at different doses and longer intervention periods, are needed to better determine the potential benefits of this dietary treatment.
Grants:	Ministerio de Economía, Industria y Competitividad PSI2016-77234-R Ministerio de Ciencia e Innovación BES-2017-081939
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Adiponectin ; Calorie restriction ; Dietary supplements ; Hypothalamus ; Leptin ; Metabolic syndrome ; White adipose tissue
Published in:	Metabolites, Vol. 13 (january 2023) , ISSN 2218-1989

DOI: 10.3390/metabo13020147
PMID: 36837766

13 p, 1.4 MB

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