Enhanced dechlorination of 1,2-dichloropropane to propene in a bioelectrochemical system mediated by Dehalogenimonas
Fernandez Verdejo, David Juan (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Cortés Garmendia, M. Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Blánquez Cano, Paqui (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Marco Urrea, Ernest (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Guisasola, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date:	2021
Abstract:	Bioelectrochemical systems (BES) are promising technologies to enhance the growth of organohalide-respiring bacteria and to treat chlorinated aliphatic hydrocarbons. In this study, two carbon-based cathodic electrode materials, a graphite brush and a carbon cloth, were used as hydrogen suppliers to couple growth of Dehalogenimonas and dechlorination of 1,2-DCP to nontoxic propene in the cathode vessel. The BES with graphite brush electrode consumed ~4000 µM 1,2-DCP during 110 days and exhibited a degradation rate 5. 6-fold higher than the maximum value obtained with the carbon cloth electrode, with a cathode potential set at −0. 7 V. Quantitative PCR confirmed that Dehalogenimonas gene copies increased by two orders of magnitude in the graphite brush BES, with an average yield of 1. 2·10 8±5·10 7 cells per µmol of 1,2-DCP degraded. The use of a pulsed voltage operation (cathode potential set at −0. 6 V for 16 h and −1. 1 V for 8 h) increased the coulombic efficiency and degradation of 1,2-DCP when compared with a continuous voltage operation of −1. 1 V. Bacterial cell aggregates were observed in the surface of the graphite brush electrodes by electron scanning microscopy, suggesting biofilm formation. This study expands the range of chlorinated compounds degradable and organohalide-respiring bacteria capable of growing in BES.
Grants:	Ministerio de Ciencia e Innovación CTM2017-91879-EXP
Note:	Altres ajuts: D. Fernández-Verdejo acknowledges a predoctoral grant from Universitat Autònoma de Barcelona (PIF 2017-2018)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	1,2-dichloropropane ; Bioelectrochemistry ; Dehalogenimonas ; Organohalide respiring bacteria ; Remediation
Published in:	Journal of hazardous materials, Vol. 416 (Aug. 2021) , art. 126234, ISSN 1873-3336

DOI: 10.1016/j.jhazmat.2021.126234
PMID: 34492987

Preprint 29 p, 763.8 KB

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