Absence of major epigenetic and transcriptomic changes accompanying an interspecific cross between peach and almond
Tomás, Carlos de (Centre de Recerca en Agrigenòmica)
Bardil, Amélie (Institut écologie et environnement (Montpelier))
Castanera, Raúl (Centre de Recerca en Agrigenòmica)
Casacuberta i Suñer, Josep M 1962- (Centre de Recerca en Agrigenòmica)
Vicient Sánchez, Carlos M. (Centre de Recerca en Agrigenòmica)

Fecha:	2022
Resumen:	Hybridization has been widely used in breeding of cultivated species showing low genetic variability, such as peach (Prunus persica). The merging of two different genomes in a hybrid often triggers a so-called "genomic shock" with changes in DNA methylation and in the induction of transposable element expression and mobilization. Here, we analysed the DNA methylation and transcription levels of transposable elements and genes in leaves of Prunus persica and Prunus dulcis and in an F1 hybrid using high-throughput sequencing technologies. Contrary to the "genomic shock" expectations, we found that the overall levels of DNA methylation in the transposable elements in the hybrid are not significantly altered compared with those of the parental genomes. We also observed that the levels of transcription of the transposable elements in the hybrid are in most cases intermediate as compared with that of the parental species and we have not detected cases of higher transcription in the hybrid. We also found that the proportion of genes whose expression is altered in the hybrid compared with the parental species is low. The expression of genes potentially involved in the regulation of the activity of the transposable elements is not altered. We can conclude that the merging of the two parental genomes in this Prunus persica x Prunus dulcis hybrid does not result in a "genomic shock" with significant changes in the DNA methylation or in the transcription. The absence of major changes may facilitate using interspecific peach x almond crosses for peach improvement.
Ayudas:	Agencia Estatal de Investigación IJC2020-045949-I Ministerio de Economía, Industria y Competitividad AGL2016-78992-R Agencia Estatal de Investigación PID2019-106374RB-I00 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad CEX2019-000902-S
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Horticulture research, Vol. 9 (May 2022) , art. uhac127, ISSN 2052-7276

DOI: 10.1093/hr/uhac127
PMID: 35928404

12 p, 1.2 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
Artículos > Artículos de investigación
Artículos > Artículos publicados