Phosphate-induced resistance to pathogen infection in Arabidopsis
Val-Torregrosa, Beatriz (Centre de Recerca en Agrigenòmica)
Bundó Barberà, Mireia (Centre de Recerca en Agrigenòmica)
Martín-Cardoso, Héctor (Centre de Recerca en Agrigenòmica)
Bach-Pages, Marcel (Centre de Recerca en Agrigenòmica)
Chiou, Tzyy-Jen (Academia Sinica. Agricultural Biotechnology Research Center)
Flors, Victor (Univesitat Jaume I. Departament de Ciències Agràries i del Medi Natural)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Fecha:	2022
Resumen:	In nature, plants are concurrently exposed to a number of abiotic and biotic stresses. Our understanding of convergence points between responses to combined biotic/abiotic stress pathways remains, however, rudimentary. Here we show that MIR399 overexpression, loss-of-function of PHOSPHATE2 (PHO2), or treatment with high phosphate (Pi) levels is accompanied by an increase in Pi content and accumulation of reactive oxygen species (ROS) in Arabidopsis thaliana. High Pi plants (e. g. , miR399 overexpressors, pho2 mutants, and plants grown under high Pi supply) exhibited resistance to infection by necrotrophic and hemibiotrophic fungal pathogens. In the absence of pathogen infection, the expression levels of genes in the salicylic acid (SA)- and jasmonic acid (JA)-dependent signaling pathways were higher in high Pi plants compared to wild-type plants grown under control conditions, which is consistent with increased levels of SA and JA in non-infected high Pi plants. During infection, an opposite regulation in the two branches of the JA pathway (ERF1/PDF1. 2 and MYC2/VSP2) occurs in high Pi plants. Thus, while pathogen infection induces PDF1. 2 expression in miR399 OE and pho2 plants, VSP2 expression is downregulated by pathogen infection in these plants. This study supports the notion that Pi accumulation promotes resistance to infection by fungal pathogens in Arabidopsis, while providing a basis to better understand interactions between Pi signaling and hormonal signaling pathways for modulation of plant immune responses. This study highlights the importance of phosphate (Pi) in regulating immune responses and hence disease resistance in Arabidopsis thaliana. Increasing Pi content by MIR399 overexpression, loss-of-function of PHOSPHATE2, or Pi treatment enhances resistance to infection by necrotrophic and hemibiotrophic fungal pathogens through modulation of SA- and JA-dependent signaling pathways.
Ayudas:	Agencia Estatal de Investigación RTI2018-101275-B-I00 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad CEX2019-000902-S Ministerio de Economía y Competitividad BES-2016-076289
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya; Research Promotion Plan from the Universitat Jaume I (UJI-B2019-2)
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Arabidopsis thaliana ; Colletotrichum higginsianum ; Immune response ; Jasmonic acid (JA) ; MicroRNA399 (miR399) ; Phosphate (Pi) ; PHOSPHATE 2 ; Plectosphaerella cucumerina ; Reactive oxygen species (ROS) ; Salicylic acid (SA)
Publicado en:	The Plant journal, Vol. 110, Issue 2 (April 2022) , p. 452-469, ISSN 1365-313X

DOI: 10.1111/tpj.15680
PMID: 35061924

18 p, 2.3 MB

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