Photoelectro-enzymatic glucose reusable biosensor by using dithienylethene mediators
Márquez, Augusto (Institut de Microelectrònica de Barcelona)
Santiago, Sara (Universitat Autònoma de Barcelona. Departament de Química)
Dominguez, Carlos (Institut de Microelectrònica de Barcelona)
Muñoz Berbel, Xavier (Institut de Microelectrònica de Barcelona)
Guirado López, Gonzalo (Universitat Autònoma de Barcelona. Departament de Química)

Date:	2020
Abstract:	In the development of colorimetric biosensors, the use of electrochromic mediators has been accepted and widely used during decades. The main drawback of these types of enzymatic substrates is the difficult recovery of the initial redox state of the molecule, which can be done electrochemically or by antioxidants addition, complicating the initially simple structure of the biosensor. those strategies are rarely followed Actually, being the disposable biosensor configuration the most extended for this detection mechanisms. Alternatively, we propose the first reported use of a diacid dithienylethene 1,2-bis(5-carboxy-2-methylthien-3-yl)cyclopentene (DTE) photoelectrochromic compound as a substrate of the horseradish peroxidase (HRP). The photoisomerization between the open (DTEo) and closed (DTEc) forms of the molecule and the respective shift in the redox potential allowed the light-induced enzymatic detection of glucose in the glucose oxidase [(GOx)]-HRP cascade system. This fast and easy control over the enzymatic substrate availability by light pulses permits a gradually consumption and the light-regeneration of the biosensor for a number of cycles. We consider the presented results transcendent in the development of reusable and light-controlled photonic biosensing systems.
Grants:	Ministerio de Economía y Competitividad TEC2014-54449-C3-1-R Ministerio de Economía y Competitividad CTQ2015-65439-R Ministerio de Economía y Competitividad BES-2015-072946
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Optical biosensor ; Photoelectrochromism ; Enzymatic mediator ; Glucose sensing ; Regenerable biosensor
Published in:	Chemistry, Vol. 26, Issue 40 (July 2020) , p. 8714-8719, ISSN 1521-3765

DOI: 10.1002/chem.202000865
PMID: 32134164

Postprint 17 p, 4.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Group in Electrochemistry, Photochemistry and Organic Reactivity (GEFRO)
Articles > Research articles
Articles > Published articles