Electrochromic biosensors based on screen-printed Prussian Blue electrodes
Aller Pellitero, Miguel (Institut de Microelectrònica de Barcelona)
Fremeau, Joséphine (Centre National de la Recherche Scientifique. Université Bourgogne Franche-Comté. Institut UTINAM)
Villa, Rosa (Institut de Microelectrònica de Barcelona)
Guirado López, Gonzalo (Universitat Autònoma de Barcelona. Departament de Química)
Lakard, Boris (Centre National de la Recherche Scientifique. Université Bourgogne Franche-Comté. Institut UTINAM)
Hihn, Jean Yves (Centre National de la Recherche Scientifique. Université Bourgogne Franche-Comté. Institut UTINAM)
del Campo, F. Javier (Institut de Microelectrònica de Barcelona)

Date:	2019
Abstract:	Prussian Blue (PB)-modified graphite screen-printed electrodes are increasingly being used in electrochemical biosensors. However, they do not allow the observation of the electrochromism of PB. This work presents the construction of PB-based, electrochromic screen-printed biosensors. Although electrically more resistive than their graphite counterparts, these new PB-based electrodes enable both the amperometric and colorimetric detection of hydrogen peroxide. This is the first time that this has been achieved using screen-printed electrodes, and we demonstrate it spectroelectrochemically on a glucose biosensor. The biosensor electrochemical performance equals that of previously reported PB/graphite electrodes, being able to detect down to 4 μM H2O2 and 54 μM glucose. At the same time, and in contrast to PB/graphite electrodes, the new PB-based electrodes afford the optical detection of these two analytes down to 1. 2 μM and 15 μM, respectively. The dynamic ranges of the glucose biosensors obtained at the PB-based electrodes are 0. 1-1 mM (amperometric) and 0. 025-2. 5 mM (Colorimetric), matching the physiological glucose concentration range in body fluids other than blood or serum.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca PROD-0000114
Note:	Altres ajuts: 2016 Leonardo grant from the BBVA Foundation
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Biosensors ; Glucose sensing ; Hydrogen peroxide detection ; Prussian Blue electrodes ; Screen-printed electrodes ; Spectroelectrochemistry
Published in:	Sensors and actuators. B, Chemical, Vol. 290 (July 2019) , p. 591-597, ISSN 1873-3077

DOI: 10.1016/j.snb.2019.03.100

Postprint 43 p, 3.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Group in Electrochemistry, Photochemistry and Organic Reactivity (GEFRO)
Articles > Research articles
Articles > Published articles