Proteogenomics of the novel Dehalobacterium formicoaceticum strain EZ94 highlights a key role of methyltransferases during anaerobic dichloromethane degradation
Wasmund, Kenneth (University of Vienna)
Trueba-Santiso, Alba (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Vicent i Huguet, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Adrian, Lorenz (Technische Universität Berlin)
Vuilleumier, Stéphane (Université de Strasbourg)
Marco Urrea, Ernest (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Fecha:	2023
Resumen:	Dichloromethane (DCM, methylene chloride) is a toxic, high-volume industrial pollutant of long-standing. Anaerobic biodegradation is crucial for its removal from contaminated environments, yet prevailing mechanisms remain unresolved, especially concerning dehalogenation. In this study, we obtained an assembled genome of a novel DCM-degrading strain, Dehalobacterium formicoaceticum strain EZ94, from a stable DCM-degrading consortium, and we analyzed its proteome during degradation of DCM. A gene cluster recently predicted to play a major role in anaerobic DCM catabolism (the mec cassette) was found. Methyltransferases and other proteins encoded by the mec cassette were among the most abundant proteins produced, suggesting their involvement in DCM catabolism. Reductive dehalogenases were not detected. Genes and corresponding proteins for a complete Wood-Ljungdahl pathway, which could enable further metabolism of DCM carbon, were also found. Unlike for the anaerobic DCM degrader "Ca. F. warabiya," no genes for metabolism of the quaternary amines choline and glycine betaine were identifed. This work provides independent and supporting evidence that mecassociated methyltransferases are key to anaerobic DCM metabolism.
Ayudas:	Ministerio de Economía y Competitividad CTM2016-75587-C2-1-R Agencia Estatal de Investigación PID2019-103989RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-14 Ministerio de Economía y Competitividad BES-2014-070817
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Dehalobacterium ; Anaerobic dichloromethane degradation ; Methyltransferases ; Wood-Ljungdahl pathway ; Shotgun proteomics
Publicado en:	Environmental Science and Pollution Research International, (June 2023), ISSN 1614-7499

DOI: 10.1007/s11356-023-28144-1
PMID: 37300728

11 p, 1.1 MB

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