Host-associated variability of the cdtABC operon, coding for the cytolethal distending toxin, in Campylobacter jejuni
Guirado, Pedro (Universitat de Barcelona)
Iglesias Torrens, Yaidelys (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Miró, Elisenda (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Navarro Risueño, Ferran (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Attolini, Camile Stephan-Otto (Institut de Ciència i Tecnologia de Barcelona)
Balsalobre Parra, Carlos (Universitat de Barcelona)
Madrid Xufré, Cristina (Universitat de Barcelona)
Universitat Autònoma de Barcelona

Date:	2022
Abstract:	Campylobacter, a major cause of food-borne gastroenteritis worldwide, colonize the gastrointestinal tract of a wide range of animals, being birds the main reservoir. The mechanisms involved in the interaction of Campylobacter with the different hosts are poorly understood. The cytolethal distending toxin, encoded in the cdtABC operon, is considered a pivotal virulence factor during human infection. Differences in the prevalence of cdtABC genes in Campylobacter isolates from three distinct origins (wild birds, broiler chickens and humans) prompted us to further characterize their allelic variability. The sequence of cdtABC is highly conserved among broiler and human isolates. A high diversity of cdtABC alleles was found among wild bird isolates, including several alleles that do not produce any functional CDT. These results suggest that specific variants of the cdtABC operon might define the host range of specific Campylobacter jejuni isolates. Moreover, our data indicate that PCR methodology is inaccurate to characterize the prevalence of the cdt genes, since negative PCR detection can be the result of divergences in the sequence used for primer design rather than indicating the absence of a specific gene.
Grants:	Ministerio de Economía y Competitividad AGL2013-45339R Agencia Estatal de Investigación PGC2018-096958-B-I00
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Allelic variability ; Campylobacter ; Cytolethal distending toxin ; PCR detection ; WGS
Published in:	Zoonoses and Public Health, Vol. 69 (september 2022) , p. 966-977, ISSN 1863-2378

DOI: 10.1111/zph.12994
PMID: 36053024

12 p, 8.1 MB

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