Composition and diversity analysis of the lung microbiome in patients with suspected ventilator-associated pneumonia
Fenn, Dominic (University of Manchester)
Abdel-Aziz, Mahmoud I. (University of Manchester)
van Oort, Pouline M. P. (University of Manchester)
Brinkman, Paul (University of Manchester)
Ahmed, Waqar M. (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Felton, Timothy (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Artigas Raventós, Antoni (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Póvoa, Pedro (New University of Lisbon)
Martin-Loeches, Ignacio (St. James's Hospital ( Dublín, Irlanda))
Schultz, Marcus J. (Amsterdam University Medical Center (UMC))
Dark, Paul (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Fowler, Stephen J. (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Bos, Lieuwe D (Amsterdam University Medical Center (UMC))
Universitat Autònoma de Barcelona

Fecha:	2022
Resumen:	Ventilator-associated pneumonia (VAP) is associated with high morbidity and health care costs, yet diagnosis remains a challenge. Analysis of airway microbiota by amplicon sequencing provides a possible solution, as pneumonia is characterised by a disruption of the microbiome. However, studies evaluating the diagnostic capabilities of microbiome analysis are limited, with a lack of alignment on possible biomarkers. Using bronchoalveolar lavage fluid (BALF) from ventilated adult patients suspected of VAP, we aimed to explore how key characteristics of the microbiome differ between patients with positive and negative BALF cultures and whether any differences could have a clinically relevant role. BALF from patients suspected of VAP was analysed using 16s rRNA sequencing in order to: (1) differentiate between patients with and without a positive culture; (2) determine if there was any association between microbiome diversity and local inflammatory response; and (3) correctly identify pathogens detected by conventional culture. Thirty-seven of 90 ICU patients with suspected VAP had positive cultures. Patients with a positive culture had significant microbiome dysbiosis with reduced alpha diversity. However, gross compositional variance was not strongly associated with culture positivity (AUROCC range 0. 66-0. 71). Patients with a positive culture had a significantly higher relative abundance of pathogenic bacteria compared to those without [0. 45 (IQR 0. 10-0. 84), 0. 02 (IQR 0. 004-0. 09), respectively], and an increased interleukin (IL)-1β was associated with reduced species evenness (r = - 0. 33, p < 0. 01) and increased pathogenic bacteria presence (r = 0. 28, p = 0. 013). Untargeted 16s rRNA pathogen detection was limited by false positives, while the use of pathogen-specific relative abundance thresholds showed better diagnostic accuracy (AUROCC range 0. 89-0. 998). Patients with positive BALF culture had increased dysbiosis and genus dominance. An increased caspase-1-dependent IL-1b expression was associated with a reduced species evenness and increased pathogenic bacterial presence, providing a possible causal link between microbiome dysbiosis and lung injury development in VAP. However, measures of diversity were an unreliable predictor of culture positivity and 16s sequencing used agnostically could not usefully identify pathogens; this could be overcome if pathogen-specific relative abundance thresholds are used. The online version contains supplementary material available at 10. 1186/s13054-022-04068-z.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Microbiome ; Next-generation sequencing ; Ventilator-associated pneumonia
Publicado en:	Critical care, Vol. 26 (july 2022) , ISSN 1466-609X

DOI: 10.1186/s13054-022-04068-z
PMID: 35794610

12 p, 1.4 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Investigación e Innovación Parc Taulí (I3PT)
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