Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy
Muñoz-Braceras, Sandra (National Institutes of Health (Estats Units d'Amèrica))
Pinal-Fernandez, Iago (Johns Hopkins University School of Medicine)
Casal-Dominguez, Maria (Johns Hopkins University School of Medicine)
Pak, Katherine (National Institutes of Health (Estats Units d'Amèrica))
Milisenda, José César (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Lu, Shajia (National Institutes of Health (Estats Units d'Amèrica))
Gadina, Massimo (National Institutes of Health (Estats Units d'Amèrica))
Naz, Faiza (National Institutes of Health (Estats Units d'Amèrica))
Gutierrez-Cruz, Gustavo (National Institutes of Health (Estats Units d'Amèrica))
Dell'Orso, Stefania (National Institutes of Health (Estats Units d'Amèrica))
Torres-Ruiz, Jiram (Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (Mèxic))
Grau-Junyent, Josep Maria (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Selva O'Callaghan, Albert (Hospital Universitari Vall d'Hebron)
Paik, Julie J. (Johns Hopkins University School of Medicine)
Albayda, Jemima (Johns Hopkins University School of Medicine)
Christopher-Stine, Lisa (Johns Hopkins University School of Medicine)
Lloyd, Thomas E. (Johns Hopkins University School of Medicine)
Corse, Andrea (Johns Hopkins University School of Medicine)
Mammen, Andrew L. (Johns Hopkins University School of Medicine)
Universitat Autònoma de Barcelona

Fecha:	2023
Resumen:	Dermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotizing myopathy (IMNM), and inclusion body myositis (IBM) are four major types of idiopathic inflammatory myopathy (IIM). Muscle biopsies from each type of IIM have unique transcriptomic profiles. MicroRNAs (miRNAs) target messenger RNAs (mRNAs), thereby regulating their expression and modulating transcriptomic profiles. In this study, 18 DM, 12 IMNM, 6 AS, 6 IBM, and 6 histologically normal muscle biopsies underwent miRNA profiling using the NanoString nCounter system. Eleven miRNAs were exclusively differentially expressed in DM compared to controls, seven miRNAs were only differentially expressed in AS, and nine miRNAs were specifically upregulated in IBM. No differentially expressed miRNAs were identified in IMNM. We also analyzed miRNA-mRNA associations to identify putative targets of differentially expressed miRNAs. In DM and AS, these were predominantly related to inflammation and cell cycle progression. Moreover, our analysis showed an association between miR-30a-3p, miR-30e-3p, and miR-199b-5p downregulation in DM and the upregulation of target genes induced by type I interferon. In conclusion, we show that muscle biopsies from DM, AS, and IBM patients have unique miRNA signatures and that these miRNAs might play a role in regulating the expression of genes known to be involved in IIM pathogenesis.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Inflammatory myopathies ; Myositis ; Mirna ; NanoString ; Ncounter
Publicado en:	Cells, Vol. 12 (september 2023) , ISSN 2073-4409

DOI: 10.3390/cells12172198
PMID: 37681930

13 p, 1.6 MB

El registro aparece en las colecciones:
Artículos > Artículos de investigación
Artículos > Artículos publicados