Characterization of the Genetic Environment of the blaVEB-4 Gene, Associated with a Transposable Region in a Proteus mirabilis Clinical Isolate
Espinal, Paula (Institut d'Investigació Biomèdica Sant Pau)
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)
Ramoneda, Laia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Flores, Manel (Institut d'Investigació Biomèdica Sant Pau)
Rivera, Alba (Institut d'Investigació Biomèdica Sant Pau)
Coll Figa, Pedro (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Navarro Risueño, Ferran (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2017
Description:	5 pàg.
Abstract:	Proteus mirabilis is the second most common cause of urinary tract infections and is also an important cause of nosocomial infections. TEM-type and CTX-M-type extended-spectrum β-lactamases (ESBLs) are the most widely distributed in this bacterial species, but minor ESBLs such as the VEB-type have also been identified. The aim of this study was to analyze the genetic environment of the blaVEB-4 gene found in a P. mirabilis clinical isolate recovered in Spain. P. mirabilis N2231 showed resistance to penicillins, cephalosporins, and aminoglycosides, remaining susceptible to imipenem, cefoxitin, β-lactamases inhibitors, and quinolones. Southern blot analysis revealed that blaVEB-4 was located in the chromosome. Analysis of the blaVEB-4 genetic context revealed a 15 kb segment 98% identical to the multidrug resistance (MDR) region of a Salmonella genomic island 1 (SGI1), which included a class 1 integron belonging to the In104 family, previously described in blaVEB-6-producing P. mirabilis VB1248. blaVEB-4 was surrounded by repeat elements, transposon Tn1721, and located on a class 1 integron containing aacA4-aadB-dfrA1-orfC genes. The blaVEB-4 gene was inserted in a complex structure of a class 1 integron, which is part of an MDR region of an SGI1, possibly involved in the mobilization of the gene and homologous recombination.
Grants:	Instituto de Salud Carlos III RD12/0015/0017 Instituto de Salud Carlos III CD15/00017
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Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Class A β-lactamases ; Transposon Tn1721 ; VEB enzymes
Published in:	Microbial Drug Resistance, Vol. 23, Issue 7 (October 2017) , p. 833-837, ISSN 1931-8448

DOI: 10.1089/mdr.2016.0262
PMID: 28304213

Postprint 17 p, 299.7 KB

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