Whole-genome analysis to describe a human adenovirus D8 conjunctivitis outbreak in a tertiary hospital
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)
del Cuerpo Casas, Margarita (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rubio, Marc (Institut d'Investigació Biomèdica Sant Pau)
Berengua, Carla (Institut d'Investigació Biomèdica Sant Pau)
Español, Montserrat (Institut d'Investigació Biomèdica Sant Pau)
Marín, Pilar (Institut d'Investigació Biomèdica Sant Pau)
Vela, Jose I. (Institut d'Investigació Biomèdica Sant Pau)
Pomar, Virginia (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Gutiérrez, Cristina (Institut d'Investigació Biomèdica Sant Pau)
Navarro Risueño, Ferran (Institut d'Investigació Biomèdica Sant Pau)
Rabella, Núria (Institut d'Investigació Biomèdica Sant Pau)

Date:	2021
Description:	6 pàg.
Abstract:	Conjunctivitis is a frequent ocular disorder caused by human adenoviruses (HAdVs). Only a few of the 45 HAdV-D species are associated with epidemic keratoconjunctivitis, including HAdV-D8. Nosocomial outbreaks due to HAdV-D8 have been rarely described, because keratoconjunctivitis cases are clinically diagnosed and treated without having to characterize the causative agent. Moreover, molecular typing is tedious when using classical techniques. In this study, a hospital outbreak of conjunctivitis caused by HAdV-D8 was characterized using the recently developed whole-genome sequencing (WGS) method. Of the 363 patients attending the Ophthalmology Department between July 13 and August 13, 2018, 36 may have acquired intrahospital conjunctivitis. Also, 11 of 22 samples sent to the Virology section were selected for WGS analysis. The WGS results revealed that 10 out of 11 HAdV-D8 strains were closely related. The remaining strain (Case 28) was more similar to a strain from an outbreak in Germany obtained from a public sequence database. WGS results showed that outbreak HAdV-D8 strains had a minimum percentage of identity of 94. 3%. WGS is useful in a clinical setting, because it avoids carrying out viral culture or specific polymerase chain reaction sequencing. The public availability of sequence reads makes it easier to compare clusters in circulation. In conclusion, WGS can play an important role in standard routines to describe viral outbreaks.
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Adenovirus ; HAdV-D8 ; Outbreak ; Whole-genome ; SDG 3 - Good Health and Well-being
Published in:	Journal of Medical Virology, Vol. 93, Issue 8 (August 2021) , p. 4840-4845, ISSN 1096-9071

DOI: 10.1002/jmv.26850
PMID: 33543795

Preprint 17 p, 410.2 KB

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