Ascitic fluid regulates the local innate immune response of patients with cirrhosis
Nieto Sáchica, Juan Camilo (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Perea, Lidia (Institut d'Investigació Biomèdica Sant Pau)
Soriano, German (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Zamora, Carlos (Institut d'Investigació Biomèdica Sant Pau)
Cantó, Elisabet (Institut d'Investigació Biomèdica Sant Pau)
Medina Dols, Aina (Institut d'Investigació Biomèdica Sant Pau)
Poca Pastor, María Antonia (Hospital Universitari Vall d'Hebron)
Sánchez, Elisabet (Instituto de Salud Carlos III)
Roman, Eva (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Julià, Germà (Universitat Autònoma de Barcelona. Departament de Medicina)
Navarro Risueño, Ferran (Institut d'Investigació Biomèdica Sant Pau)
Gely, Cristina (Institut d'Investigació Biomèdica Sant Pau)
Alvarado-Tapias, Edilmar (Institut d'Investigació Biomèdica Sant Pau)
Guarner-Argente, Carlos (Institut d'Investigació Biomèdica Sant Pau)
Juarez, Candido (Institut d'Investigació Biomèdica Sant Pau)
Vidal, Silvia (Institut d'Investigació Biomèdica Sant Pau)

Fecha:	2018
Descripción:	9 pàg.
Resumen:	Ascitic neutrophils from cirrhotic patients with spontaneous bacterial peritonitis (SBP) exhibit an impaired oxidative burst that could facilitate bacterial infection. However, the influence of the cell-free ascitic fluid of these patients on neutrophil function has not been investigated. To analyze this influence, we determined the ascitic levels of cytokines, resistin, and lactoferrin and their association with neutrophil function, disease severity score, and SBP resolution. We analyzed NETosis induction by microscopy and oxidative burst by the flow cytometry of healthy neutrophils cultured in ascitic fluid from cirrhotic patients with sterile ascites (SA) and with SBP before and after antibiotic treatment. Resistin, IL-6, IL-1 receptor antagonist, IL-1β, and lactoferrin levels were measured in ascitic fluids and supernatants of cultured neutrophils and PBMCs by ELISA. Upon stimulation, healthy neutrophils cultured in SBP ascitic fluid produced lower NETosis and oxidativeburst than those cultured in SA. Ascitic resistin levels were negatively correlated with NETosis, oxidative burst, and ascitic glucose levels; and positively correlated with the model for end-stage liver disease score. After an E. coli or TNF-α stimulus, neutrophils were the major resistin producers. Resistin indirectly reduced the oxidative burst of neutrophils and directly reduced the inflammatory phenotype of monocytes and TNF-α production. Bacterial-induced resistin production can down-regulate the inflammatory response of macrophages and neutrophil function in ascitic fluid. Consequently, this down-regulation may jeopardize the elimination of bacteria that translocate to ascitic fluid in patients with cirrhosis.
Ayudas:	Instituto de Salud Carlos III PI09/00357 Instituto de Salud Carlos III PI09/00132
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	NETosis ; Oxidative burst ; Resistin ; Spontaneous bacterial peritonitis
Publicado en:	Journal of Leukocyte Biology, Vol. 104, Issue 4 (October 2018) , p. 833-841, ISSN 1938-3673

DOI: 10.1002/JLB.3A0218-072R
PMID: 29851144

Postprint 33 p, 4.3 MB

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