Differences in LexA regulon structure among Proteobacteria through in vivo assisted comparative genomics
Erill, Ivan (Centro Nacional de Microelectrónica (Espanya))
Jara Ramírez, Mónica (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Escribano, Marcos (Centro Nacional de Microelectrónica (Espanya))
Campoy Sánchez, Susana (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Barbé García, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Salvador, Noelia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2004
Abstract:	The LexA regulon encompasses an ensemble of genes involved in preserving cell viability under massive DNA damage and is present in most bacterial phyla. Up to date, however, the scope of this network had only been assessed in the Gamma Proteobacteria. Here, we report the structure of the LexA regulon in the Alpha Proteobacteria, using a combined approach that makes use of in vitro and in vivo techniques to assist and validate the comparative genomics in silico methodology. This leads to the first experimentally validated description of the LexA regulon in the Alpha Proteobacteria, and comparison of regulon core structures in both classes suggests that a least common multiple set of genes ( recA , ssb , uvrA and ruvCAB ) might be a defining property of the Proteobacteria LexA network.
Grants:	Ministerio de Educación y Ciencia BMC2001-2065 Ministerio de Educación y Ciencia BFM2004-02768/BMC Agència de Gestió d'Ajuts Universitaris i de Recerca 2001/SGR-206
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Published in:	Nucleic Acids Research, Vol. 32, issue 22 (Nov. 2004) , p. 6617-6626, ISSN 0305-1048

DOI: 10.1093/nar/gkh996
PMID: 15604457

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