Presumed pseudo-Pelger-Huët anomaly and basophilia secondary to chronic lymphocytic leukemia in a dog
Martínez-Caro, Javier (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Agulla, Beatriz (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Viñeta, Clàudia (Universitat Autònoma de Barcelona. Hospital Clínic Veterinari)
Roura, Xavier (Universitat Autònoma de Barcelona. Hospital Clínic Veterinari)
Mesalles Naranjo, Montse (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2024
Abstract:	A 10-year-old neutered male Maltese dog was presented for an investigation of lymphocytosis. The dog was up-to-date on vaccinations and deworming. Physical examination did not reveal any significant abnormalities. A complete blood cell count (CBC) showed mild leukocytosis with moderate lymphocytosis, basophilia, and moderate neutropenia, but no significant left shift or toxic change. Serum biochemistry and urinalysis were unremarkable. All performed tests for infectious agents common in this geographical region were negative. No significant abnormalities were found on abdominal ultrasound examination. Multiparametric flow cytometry of peripheral blood showed a CD8 T-cell lymphocytosis, and PCR for antigen receptor rearrangement revealed a clonal expansion of the T-cell receptor gamma chain genes. A clinical diagnosis of chronic lymphocytic leukemia (CLL) was made, and follow-up was recommended. On Day 48 post-presentation, the CBC showed mild non-regenerative anemia (NRA), moderate leucocytosis due to moderate to marked lymphocytosis, basophilia, and a marked increase in hyposegmented neutrophils with mild toxic change in the absence of neutrophilia or neutropenia. Treatment with chlorambucil and prednisolone was initiated. On Days 87 and 197 post-presentation, the CBC showed mild NRA, with progressively decreasing numbers of hyposegmented neutrophils. The dog remained without clinical signs. Basophilia and probable pseudo-Pelger-Huët anomaly were possibly secondary to CLL. To the authors' knowledge, this is the first report of these two hematologic conditions secondary to CLL in dogs. Recognition of a pseudo-Pelger-Huët anomaly is clinically relevant to avoid misinterpretation as a marked left shift due to severe inflammation and prevent unnecessary urgent therapeutic actions.
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Basophils ; Canine ; Flow cytometry ; Hematology ; Hyposegmented granulocytes ; Lymphoproliferative disease
Published in:	Veterinary clinical pathology, 2024 , ISSN 1939-165X

DOI: 10.1111/vcp.13347
PMID: 38622430

7 p, 2.1 MB

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