Biocontrol potential of six Heterorhabditis bacteriophora strains isolated in the Azores Archipelago
Beltrí, Rubén (Universidade Dos Açores. Centro de Biotecnologia Dos Açores)
Monteiro, Hugo R. (Universidade Dos Açores. Centro de Biotecnologia Dos Açores)
Toubarro, Duarte (Universidade Dos Açores. Centro de Biotecnologia Dos Açores)
Simões, N. (Universidade Dos Açores. Centro de Biotecnologia Dos Açores)
Garriga, Anna (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)

Fecha:	2024
Resumen:	Entomopathogenic nematodes (EPNs) are closely associated with Popillia japonica and potentially used as their biological control agents, although field results proved inconsistent and evoked a continual pursuit of native EPNs more adapted to the environment. Therefore, we surveyed the Azorean Archipelago to isolate new strains of Heterorhabditis bacteriophora and to evaluate their virulence against the model organism Galleria mellonella under laboratory conditions. Six strains were obtained from pasture and coastal environments and both nematode and symbiont bacteria were molecularly identified. The bioassays revealed that Az172, Az186, and Az171 presented high virulence across the determination of a lethal dose (LD50) and short exposure time experiments with a comparable performance to Az29. After 72 hours, these virulent strains presented a mean determination of a lethal dose of 11 infective juveniles cm, a lethal time (LT50) of 34 hours, and achieved 40% mortality after an initial exposure time of only 60 minutes. Az170 exhibited an intermediate performance, whereas Az179 and Az180 were classified as low virulent strains. However, both strains presented the highest reproductive potential with means of 1700 infective juveniles/mg of larvae. The bioassays of the native EPNs obtained revealed that these strains hold the potential to be used in biological control initiatives targeting P. japonica because of their high virulence and locally adapted to environmental conditions.
Ayudas:	European Commission 861852
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Entomopathogenic nematodes ; Biological control ; Native isolates ; Virulence ; Phylogeny
Publicado en:	Journal of helminthology, Vol. 98 (May 2024) , art. e43, ISSN 1475-2697

DOI: 10.1017/S0022149X24000336

10 p, 838.8 KB

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