@article{ddd.uab.cat:293742,
      author = {Martín-Pérez, Joan and Villacorta, Aliro and Banaei, Gooya and
               Morataya-Reyes, Michelle and Tavakolpournegari, Alireza and
               Marcos Dauder, Ricardo and Hernández Bonilla, Alba and
               García-Rodríguez, Alba},
       title = {Hazard assessment of nanoplastics is driven by their surface-
               functionalization. Effects in human-derived primary endothelial
               cells},
     journal = {Science of the total environment},
        year = {2024},
      volume = {934},
       pages = {173236--},
       month = {7},
        note = {Altres ajuts: acords transformatius de la UAB},
    abstract = {During plastic waste degradation into micro/nanoplastics (MNPLs)
               their physicochemical characteristics including surface
               properties (charge, functionalization, biocorona, etc.) can
               change, potentially affecting their biological effects. This
               paper focuses on the surface functionalization of MNPLs to
               determine if it has a direct impact on the toxicokinetic and
               toxicodynamic interactions in human umbilical vein endothelial
               cells (HUVECs), at different exposure times. Pristine polystyrene
               nanoplastics (PS-NPLs), as well as their carboxylated (PS-C-NPLs)
               and aminated (PS-A-NPLs) forms, all around 50 nm, were used in a
               wide battery of toxicological assays. These assays encompassed
               evaluations on cell viability, cell internalization, induction of
               intracellular reactive oxygen species (iROS), and genotoxicity.
               The experiments were conducted at a concentration of 100 μg/mL,
               chosen to ensure a high internalization rate across all
               treatments while maintaining a sub-toxic concentration. Our
               results show that all PS-NPLs are internalized by HUVECs, but the
               internalization dynamic depends on the particle's
               functionalization. PS-NPLs and PS-C-NPLs internalization modify
               the morphology of the cell increasing its inner
               complexity/granularity. Regarding cell toxicity, only PS-A-NPLs
               reduced cell viability. Intracellular ROS was induced by the
               three different PS-NPLs but at different time points. Genotoxic
               damage was induced by the three PS-NPLs at short exposures (2 h),
               but not for PS-C-NPLs at 24 h. Overall, this study suggests that
               the toxicological effects of PSNPLs on HUVEC cells are surface-
               dependent, highlighting the relevance of using human-derived
               primary cells as a target.},
         doi = {10.1016/j.scitotenv.2024.173236},
         url = {https://ddd.uab.cat/record/293742},
}