Metabolic Pathway Modeling in Muscle of Male Marathon Mice (DUhTP) and Controls (DUC)-A Possible Role of Lactate Dehydrogenase in Metabolic Flexibility
Brenmoehl, Julia (Institute of Genome Biology, Research Institute for Farm Animal Biology)
Brosig, Elli (University Medicine Rostock. Department of Neurology)
Trakooljul, Nares (Institute of Genome Biology, Research Institute for Farm Animal Biology)
Walz, Christina (Institute of Genome Biology, Research Institute for Farm Animal Biology)
Ohde, Daniela (Institute of Genome Biology, Research Institute for Farm Animal Biology)
Noce, Antonia (Centre de Recerca en Agrigenòmica)
Walz, Michael (Institute of Genome Biology, Research Institute for Farm Animal Biology)
Langhammer, Martina (Institute of Genetics and Biometry, Research Institute for Farm Animal Biology. Lab Animal Facility)
Petkov, Stefan (Institute of Muscle Biology and Growth, Research Institute for Farm Animal Biology)
Röntgen, Monika (Institute of Muscle Biology and Growth, Research Institute for Farm Animal Biology)
Maak, Steffen (Institute of Muscle Biology and Growth, Research Institute for Farm Animal Biology)
Galuska, Christina E. (Research Institute for Farm Animal Biology. Core Facility Metabolomics)
Fuchs, Beate (Research Institute for Farm Animal Biology. Core Facility Metabolomics)
Kuhla, Björn (Institute of Nutrition, Research Institute for Farm Animal Biology)
Ponsuksili, Siriluck (Institute of Genome Biology, Research Institute for Farm Animal Biology)
Wimmers, Klaus (Institute of Genome Biology, Research Institute for Farm Animal Biology)
Hoeflich, Andreas (Institute of Genome Biology, Research Institute for Farm Animal Biology)

Fecha:	2023
Resumen:	In contracting muscles, carbohydrates and fatty acids serve as energy substrates; the predominant utilization depends on the workload. Here, we investigated the contribution of non-mitochondrial and mitochondrial metabolic pathways in response to repeated training in a polygenic, paternally selected marathon mouse model (DUhTP), characterized by exceptional running performance and an unselected control (DUC), with both lines descended from the same genetic background. Both lines underwent three weeks of high-speed treadmill training or were sedentary. Both lines' muscles and plasma were analyzed. Muscle RNA was sequenced, and KEGG pathway analysis was performed. Analyses of muscle revealed no significant selection-related differences in muscle structure. However, in response to physical exercise, glucose and fatty acid oxidation were stimulated, lactate dehydrogenase activity was reduced, and lactate formation was inhibited in the marathon mice compared with trained control mice. The lack of lactate formation in response to exercise appears to be associated with increased lipid mobilization from peripheral adipose tissue in DUhTP mice, suggesting a specific benefit of lactate avoidance. Thus, results from the analysis of muscle metabolism in born marathon mice, shaped by 35 years (140 generations) of phenotype selection for superior running performance, suggest increased metabolic flexibility in male marathon mice toward lipid catabolism regulated by lactate dehydrogenase.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Glycogen ; Lactate ; Pathway analysis ; Skeletal muscle ; Treadmill training
Publicado en:	Cells, Vol. 12, Issue 15 (July 2023) , art. 1925, ISSN 2073-4409

DOI: 10.3390/cells12151925
PMID: 37566003

21 p, 2.1 MB

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