Transcriptional Evaluation of Neuropeptides, Hormones, and Tissue Repair Modulators in the Skin of Gilthead Sea Bream (Sparus aurata L.) Subjected to Mechanical Damage
Piñera-Moreno, Rocío (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Reyes-López, Felipe E. (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Goldstein, Merari (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Santillán-Araneda, María Jesús (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Robles-Planells, Bárbara (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Arancibia-Carvallo, Camila (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Vallejos Vidal, Eva Carolina (Universidad de Santiago de Chile. Centro de Biotecnología Acuícola)
Cuesta, Alberto (University of Murcia. Department of Cell Biology and Histology)
Esteban, María Ángeles (University of Murcia. Department of Cell Biology and Histology)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Fecha:	2024
Resumen:	The skin of bony fish is the first physical barrier and is responsible for maintaining the integrity of the fish. Lesions make the skin vulnerable to potential infection by pathogens present in the aquatic environment. In this way, wound repair has barely been studied in gilthead sea bream. Thus, this study investigated the modulation of peripheral neuro-endocrine and tissue repair markers at the transcriptional level in the skin of teleost fish subjected to mechanical damage above or below the lateral line (dorsal and ventral lesions, respectively). Samples were evaluated using RT-qPCR at 2-, 4-, and 20-days post-injury. Fish with a ventral lesion presented a trend of progressive increase in the expressions of corticotropin-releasing hormone (crh), pro-opiomelanocortin-A (pomca), proenkephalin-B (penkb), cholecystokinin (cck), oxytocin (oxt), angiotensinogen (agt), and (less pronounced) somatostatin-1B (sst1b). By contrast, fish with a dorsal lesion registered no significant increase or biological trend for the genes evaluated at the different sampling times. Collectively, the results show a rapid and more robust response of neuro-endocrine and tissue repair markers in the injuries below than above the lateral line, which could be attributable to their proximity to vital organs.
Ayudas:	Agencia Estatal de Investigación PID2020-117557RB-C21 Agencia Estatal de Investigación PID2020-113637RB-C21
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Sparus aurata ; Skin wound ; Tissue repair ; Stress ; Neuro-immuno-endocrine axis ; Immune system ; Aquaculture
Publicado en:	Animals, Vol. 14, Issue 12 (June 2024) , art. 1815, ISSN 2076-2615

DOI: 10.3390/ani14121815
PMID: 38929434

17 p, 2.7 MB

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