Limited Emergence of Salmonella enterica Serovar Infantis Variants with Reduced Phage Susceptibility in PhagoVet-Treated Broilers
Sevilla-Navarro, Sandra (Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana)
Otero Carrera, Jennifer (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
López Pérez, Júlia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Torres-Boncompte, Jan (Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana)
Prucha, Tiago (VETWORKS BVBA)
Gussem, Maarten De (VETWORKS BVBA)
Silva, Daniela (ALS Life Sciences Portugal)
Burgan, Julia (ALS Life Sciences Portugal)
Catalá-Gregori, Pablo (Centro de Calidad Avícola y Alimentación Animal de la Comunidad Valenciana)
Cortés Garmendia, M. Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Llagostera Casas, Montserrat (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2024
Abstract:	Salmonella enterica serovar Infantis (S. Infantis) poses a growing issue in the poultry sector, with phage-based products emerging as a safe and effective control measure. This study investigated the emergence of reduced-phage-susceptibility variants (RPSV) of S. Infantis in PhagoVet-treated broilers, given that RPSV could undermine phage treatment efficacy. The bacteriophages in the PhagoVet product were characterized using transmission electron microscopy (TEM), genome sequencing, and infection profiling. Furthermore, two broiler trials were conducted: a challenge group (T1) and a challenge-and-treated group (T2). The S. Infantis infective dose was set at 10 4 and 10 6 colony-forming units (CFUs) per animal, with PhagoVet administration at 10 6 and 10 8 plaque-forming units (PFUs) per animal, in Trials 1 and 2, respectively. The results revealed that the four PhagoVet bacteriophages belonged to different genera. PhagoVet evidenced broad-spectrum efficacy against 271 strains representing 18 Salmonella serovars. In Trial 1, PhagoVet reduced bacterial counts in feces to nearly undetectable levels by day 42, with no RPSV detected. However, in Trial 2, three and five RPSVs were detected in feces and ceca, respectively. Consequently, PhagoVet demonstrated efficacy against S. Infantis in broilers, and the potential impact of RPSV is deemed unlikely to compromise its efficacy.
Grants:	European Commission 820523 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00646 Agencia Estatal de Investigación PID2020-117708GB-I00
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bacteriophage ; Broilers ; PhagoVet ; Reduced phage susceptibility ; Salmonella Infantis
Published in:	Animals, Vol. 14, Issue 16 (August 2024) , art. 2352, ISSN 2076-2615

DOI: 10.3390/ani14162352
PMID: 39199885

12 p, 955.6 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles