Pharmacological characterization of alpha adrenoceptor-mediated motor responses in the rat colon
Traserra, Sara (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Grao, Marc (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Trujillo, Sonia (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Jiménez Altayó, Francesc (Universitat Autònoma de Barcelona. Departament de Farmacologia, de Terapèutica i de Toxicologia)
Vergara, Patri (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Jiménez Farrerons, Marcel (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Fecha:	2024
Resumen:	Background: Inhibitory neuromuscular transmission in the gastrointestinal tract is mediated by intrinsic nitrergic and purinergic neurons. Purines activate G protein-coupled receptor P2Y receptors, increasing intracellular Ca that activates small conductance calcium-activated potassium (SK) channels. Little is known about the effect of adrenergic receptor activation on intestinal smooth muscle. In vascular tissue, stimulation of α-adrenoceptors causes smooth muscle contraction, while their effect on intestinal tissue is poorly understood. This study aimed to pharmacologically characterize the effect of α-adrenoceptor activation in the rat colon, which shares similar inhibitory pathways to the human colon. Methods: Muscle bath experiments were performed with the rat proximal, mid, and distal colon oriented both circularly and longitudinally. Results: The α-adrenoceptor agonist phenylephrine (PE) (10-10 M) evoked concentration-dependent relaxations of the intestinal smooth muscle from all regions and orientations. However, in the mid-circular colon at low PE concentrations, a contraction sensitive to 10 M phentolamine (non-selective α-adrenoceptor blocker), the neural blocker tetrodotoxin (TTX; 10 M), and atropine (10 M) was recorded. PE-induced relaxations were insensitive to TTX (10 M) and the nonselective β-adrenoceptor blocker propranolol (10 M). In contrast, PE-induced relaxations were blocked by phentolamine (10 M), prazosin (10 M) (α-adrenoceptor blocker), and RS17053 (10 M) (α-blocker), but not by yohimbine (10 M) (α-adrenoceptor blocker). Apamin (10 M), a SK channel blocker, abolished PE-induced relaxations. Conclusions: Contractile responses in the circular muscle of the mid colon could be attributed to α-adrenoceptors located on enteric cholinergic neurons. Stimulation of α-adrenoreceptors activates SK channels to cause smooth muscle relaxation, which constitutes a signaling pathway that shares similarities with P2Y receptors.
Ayudas:	Agencia Estatal de Investigación PID2020-113634RB-C22
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Alpha adrenoceptors ; Apamin ; Colon ; Phenylephrine ; Relaxation
Publicado en:	Neurogastroenterology & motility, 2024 , ISSN 1365-2982

DOI: 10.1111/nmo.14921
PMID: 39344996

10 p, 7.0 MB

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