Methodological approaches in vitrification : Enhancing viability of bovine oocytes and in vitro-produced embryos
Mogas Amorós, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Garcia Martinez, Tania (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Martínez-Rodero, Iris (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2024
Resumen:	Cryopreservation of bovine oocytes and embryos is essential for long-term preservation and widespread distribution of genetic material, particularly in bovine in vitro embryo production, which has witnessed substantial growth in the past decade due to advancements in reproductive biotechnologies. Among current cryopreservation methods, vitrification has emerged as the preferred cryopreservation technique over slow freezing for preserving oocytes and in vitro-produced (IVP) embryos, as it effectively addresses membrane chilling injury and ice crystal formation. Nonetheless, challenges remain and a simple and robust vitrification protocol that guarantees the efficiency and viability after warming has not yet been developed. Furthermore, although slow cooling can easily be adapted for direct transfer, an easier and more practical vitrification protocol for IVP embryos is required to allow the transfer of IVP embryos on farms using in-straw dilution. In addition, the susceptibility of bovine oocytes and embryos to cryoinjuries highlights the need for novel strategies to improve their cryotolerance. This manuscript examines various methodological approaches for increasing the viability of bovine oocytes and IVP embryos during vitrification. Strategies such as modifying lipid content or mitigating oxidative damage have shown promise in improving cryotolerance. Additionally, mathematical modelling of oocyte and embryo membrane permeability has facilitated the rational design of cryopreservation protocols, optimizing the exposure time and concentration of cryoprotectants to reduce cytotoxicity.
Ayudas:	Ministerio de Ciencia e Innovación AGL2016-79802-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1229 Agencia Estatal de Investigación BES-2017-081962 Ministerio de Ciencia e Innovación PID2020-116531RB-I002017
Nota:	Altres ajuts: acords transformatius de la UAB
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article de revisió ; recerca ; Versió publicada
Materia:	Blastocoel collapse ; Lipid content ; Mathematical modelling ; Membrane permeability ; Oxidative damage
Publicado en:	Reproduction in domestic animals, Vol. 59 Núm. S3 (2024) , p. e14623, ISSN 1439-0531

DOI: 10.1111/rda.14623
PMID: 39396876

17 p, 1.3 MB

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