Evaluation of triple negative breast cancer with heterogeneous immune infiltration
Quintana, Angela (Vall d'Hebron Institut d'Oncologia)
Arenas, Enrique J (Vall d'Hebron Institut d'Oncologia)
Bernadó Morales, Cristina (Vall d'Hebron Institut d'Oncologia)
Navarro, José Fernández (Vall d'Hebron Institut d'Oncologia)
González, Jonatan (Vall d'Hebron Institut d'Oncologia)
Esteve-Codina, Anna (Universitat Pompeu Fabra)
Moliné, Teresa (Hospital Universitari Vall d'Hebron)
Martí, Mercè (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Curigliano, Giuseppe (University of Milano)
Schmid, Peter (Queen Mary University London)
Peg, Vicente (Universitat Autònoma de Barcelona. Departament de Medicina)
Arribas, Joaquín V (Institut Hospital del Mar d'Investigacions Mèdiques)
Cortés Castán, Javier (Vall d'Hebron Institut d'Oncologia)

Fecha:	2023
Descripción:	8 pàg.
Resumen:	Introduction: Tumor infiltrating lymphocytes (TILs) are known to be a prognostic and predictive biomarker in breast cancer, particularly in triple negative breast cancer (TNBC) patients. International guidelines have been proposed to evaluate them in the clinical setting as a continuous variable, without a clear defined cut-off. However, there are scenarios where the immune infiltration is heterogeneous that some areas of the patient's tumour have high numbers of TILs while other areas completely lack them. This spontaneous presentation of a heterogeneous immune infiltration could be a great opportunity to study why some tumours present TILs at diagnosis but others do not, while eliminating inter patient's differences. Methods: In this study, we have identified five TNBC patients that showed great TIL heterogeneity, with areas of low (≤5%) and high (≥50%) numbers of TILs in their surgical specimens. To evaluate immune infiltration heterogeneity, we performed and analyzed bulk RNA-sequencing in three independent triplicates from the high and low TIL areas of each patient. Results: Gene expression was homogeneous within the triplicates in each area but was remarkable different between TILs regions. These differences were not only due to the presence of TILs as there were other non-inflammatory genes and pathways differentially expressed between the two areas. Discussion: This highlights the importance of intratumour heterogeneity driving the immune infiltration, and not patient's characteristics like the HLA phenotype, germline DNA or immune repertoire.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Desconeguda
Documento:	Article ; recerca ; Versió publicada
Materia:	SDG 3 - Good Health and Well-being
Publicado en:	Frontiers in immunology, Vol. 14 (2023) , p. 1149747, ISSN 1664-3224

DOI: 10.3389/fimmu.2023.1149747
PMID: 37215143

8 p, 6.3 MB

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