Experimental Setting for Applying Mechanical Stimuli to Study the Endothelial Response of Ex Vivo Vessels under Realistic Pathophysiological Environments
Osuna, Ana (Universitat de Barcelona)
Ulldemolins, Anna (Universitat de Barcelona)
Sanz-Fraile, Hector (Universitat de Barcelona)
Otero, Jorge (Universitat de Barcelona)
Farré, Núria (Universitat Autònoma de Barcelona. Departament de Medicina)
Farré, Ramon (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Almendros, Isaac (Institut d'Investigacions Biomèdiques August Pi i Sunyer)

Date:	2021
Abstract:	This paper describes the design, construction and testing of an experimental setting, making it possible to study the endothelium under different pathophysiological conditions. This novel experimental approach allows the application of the following stimuli to an ex vivo vessel in a physiological bath: (a) a realistic intravascular pressure waveform defined by the user; (b) shear stress in the endothelial layer since, in addition to the pressure waveform, the flow through the vessel can be independently controlled by the user; (c) conditions of hypo/hyperoxia and hypo/hypercapnia in an intravascular circulating medium. These stimuli can be applied alone or in different combinations to study possible synergistic or antagonistic effects. The setting performance is illustrated by a proof of concept in an ex vivo rabbit aorta. The experimental setting is easy to build by using very low-cost materials widely available. Online Supplement files provide all the technical information (e. g. , circuits, codes, 3D printer drivers) following an open-source hardware approach for free replication.
Grants:	Agencia Estatal de Investigación SAF2017-85574-R Agencia Estatal de Investigación PGC2018-097323-A-I00 Agencia Estatal de Investigación PID2019-108958RB-I00
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bioreactor ; Vessel mechanics ; Experimental physiology ; Mechanical stimuli ; Hypoxia ; Open-source hardware
Published in:	Life, Vol. 11 (july 2021) , ISSN 2075-1729

DOI: 10.3390/life11070671
PMID: 34357043

9 p, 1.9 MB

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