Simultaneous entry as an adaptation to virulence in a novel satellite-helper system infecting Streptomyces species
deCarvalho, Tagide (University of Maryland Baltimore County)
Mascolo, Elia (University of Maryland Baltimore County. Department of Biological Sciences)
Caruso, Steven (University of Maryland Baltimore County. Department of Biological Sciences)
López Pérez, Júlia (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Weston-Hafer, Kathleen (Washington University in St. Louis. Department of Biology)
Shaffer, Christopher (Washington University in St. Louis. Department of Biology)
Erill, Ivan (Universitat Autònoma de Barcelona. Departament d'Enginyeria de la Informació i de les Comunicacions)

Date:	2023
Abstract:	Satellites are mobile genetic elements that are dependent upon the replication machinery of their helper viruses. Bacteriophages have provided many examples of satellite nucleic acids that utilize their helper morphogenic genes for propagation. Here we describe two novel satellite-helper phage systems, Mulch and Flayer, that infect Streptomyces species. The satellites in these systems encode for encapsidation machinery but have an absence of key replication genes, thus providing the first example of bacteriophage satellite viruses. We also show that codon usage of the satellites matches the tRNA gene content of the helpers. The satellite in one of these systems, Flayer, does not appear to integrate into the host genome, which represents the first example of a virulent satellite phage. The Flayer satellite has a unique tail adaptation that allows it to attach to its helper for simultaneous co-infection. These findings demonstrate an ever-increasing array of satellite strategies for genetic dependence on their helpers in the evolutionary arms race between satellite and helper phages.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	The ISME Journal, Vol. 17, Issue 12 (December 2023) , p. 2381-2388, ISSN 1751-7370

DOI: 10.1038/s41396-023-01548-0
PMID: 37907733

8 p, 2.5 MB

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