The Use of the Nitroblue Tetrazolium Test in Blood Granulocytes for Discriminating Bacterial and Non-Bacterial Neutrophilic Dermatitis
García, Marina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Martínez-Flórez, Icíar (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Solano Gallego, Laia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
García, Núria (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ordeix, Laura (Ordeix i Esteve) (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Fecha:	2024
Resumen:	The nitroblue tetrazolium (NBT) reduction test measures metabolic activity in cells. This study aimed to compare reactive oxygen species (ROS) production in neutrophils from healthy dogs, dogs with superficial pyoderma, and dogs with sterile neutrophilic pustular dermatitis. Twenty-eight dogs were divided into three groups, and their blood was tested using the NBT reduction method. The results showed that dogs with sterile neutrophilic dermatitis had higher NBT rates, especially if they tested positive for Leishmania spp. The NBT test may help identify systemic neutrophil activation in immune-mediated diseases, but it was less effective in distinguishing between healthy dogs and those with superficial pyoderma. This study aimed to evaluate differences in the production of reactive oxygen species (ROS) by peripheral blood neutrophils in healthy dogs, dogs with superficial pyoderma, and dogs with sterile neutrophilic dermatitis using the nitroblue tetrazolium (NBT) reduction test. Additionally, the study assessed the potential of the NBT reduction test as a diagnostic tool to differentiate between these clinical conditions. A total of 28 dogs were divided into three groups: healthy (n = 10), superficial pyoderma (n = 10), and sterile neutrophilic dermatitis (n = 8). The results showed significantly higher neutrophil activation in dogs with sterile neutrophilic dermatitis (19 ± 10%) compared with healthy dogs (7. 3 ± 5%) (p < 0. 01) and dogs with superficial pyoderma (10 ± 2. 8%) (p < 0. 05). Dogs with sterile neutrophilic dermatitis and a high positive result for Leishmania spp. antibodies (n = 3) exhibited even higher NBT reduction rates (30 ± 4. 6%) compared with seronegative dogs with sterile neutrophilic dermatitis (n = 5) (13 ± 5. 3%) (p < 0. 05). These findings suggest that the NBT reduction test could be useful for detecting systemic neutrophil activation in sterile neutrophilic dermatitis, especially when associated with Leishmania infection. However, no significant differences were observed between healthy dogs and those with superficial pyoderma, indicating that the test may not be effective in detecting systemic neutrophil activation in superficial bacterial infections.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	NBT ; Dog ; Neutrophil ; Superficial pyoderma ; Sterile neutrophilic dermatitis ; Oxidative metabolism
Publicado en:	Veterinary sciences, Vol. 11 (december 2024) , ISSN 2306-7381

DOI: 10.3390/vetsci11120634
PMID: 39728975

7 p, 690.0 KB

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