Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts
Rubio Lorente, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
El Yamani, Naouale (NILU-Norwegian Institute for Air Research)
Kažimirova, Alena (Slovak Medical University)
Dusinska, Maria (NILU-Norwegian Institute for Air Research)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2016
Description:	6 pàg.
Abstract:	Although there is an important set of data showing potential genotoxic effects of nanomaterials (NMs) at the DNA (comet assay) and chromosome (micronucleus test) levels, few studies have been conducted to analyze their potential mutagenic effects at gene level. We have determined the ability of multi-walled carbon nanotubes (MWCNT, NM401), to induce mutations in the HPRT gene in Chinese hamster lung (V79) fibroblasts. NM401, characterized in the EU NanoGenotox project, were further studied within the EU Framework Programme Seven (FP7) project NANoREG. From the proliferation assay data we selected a dose-range of 0. 12 to 12 mu g/cm(2) At these range we have been able to observe significant cellular uptake of MWCNT by using transmission electron microscopy (TEM), as well as a concentration-dependent induction of intracellular reactive oxygen species. In addition, a clear concentration-dependent increase in the induction of HPRT mutations was also observed. Data support a potential genotoxic/ carcinogenic risk associated with MWCNT exposure.
Grants:	European Commission 310584 European Commission 262163
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Genotoxicity ; HPRT gene ; Multi-walled carbon nanotubes ; Ros ; V79 cells
Published in:	Environmental research, Vol. 146 (April 2016) , p. 185-190, ISSN 1096-0953

DOI: 10.1016/j.envres.2016.01.004
PMID: 26774957

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