Bovine fecal biomarkers of intestinal inflammatory process : Calprotectin and lactoferrin, a comparative study
Saco Rodriguez, Yolanda (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Crusellas Villorbina, Núria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Peña, Raquel (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Pato, Raquel (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Marti, Sonia (Institut de Recerca i Tecnologia Agroalimentàries)
Pisoni, Lucía (Institut de Recerca i Tecnologia Agroalimentàries)
Devant Guille, Maria (Institut de Recerca i Tecnologia Agroalimentàries)
Pelegrí-Pineda, Anna (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Date:	2025
Description:	3 pàg.
Abstract:	Fecal lactoferrin and fecal calprotectin have been proposed as biomarkers of intestinal inflammation in several animal species. The main objectives of this work were to validate an analytical procedure for the measurement of lactoferrin in calf feces, to study the correlation between lactoferrin and calprotectin concentrations, and to evaluate the influence of fecal water content in the determination of these proteins. This knowledge is essential for effectively using these biomarkers in young calves exposed to inflammatory gastrointestinal diseases. Seventy-eight male Holstein dairy calves between two and three weeks of age were included in the study. Lactoferrin was determined with a bovine milk lactoferrin ELISA kit and calprotectin was measured using a human immunoturbidimetric method previously validated in bovine feces. Analytical validation of the lactoferrin assay achieved good results, with intra and inter assay CV < 10 %, recovery rates between 80 and 120 %, and optimal linearity under dilution. A robust correlation was observed between fecal calprotectin concentrations in dry and wet feces (r = 0. 903), while a moderate correlation was observed for fecal lactoferrin concentrations (r = 0. 648). Correlation between both biomarkers was moderate in fresh feces (r = 0. 514) as well as in dry feces (r = 0. 561). In conclusion, the lactoferrin ELISA kit is valid for its use with calf fecal samples, both biomarkers showed a moderate correlation between them, and fecal lactoferrin concentration is more influenced by feces moisture than fecal calprotectin concentration.
Grants:	Agencia Estatal de Investigación PID2019-104021RB-I00
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biomarker ; Bovine ; Calprotectin ; Feces ; Lactoferrin ; Validation
Published in:	Research in veterinary science, Vol. 183 (February 2025) , ISSN 1532-2661

DOI: 10.1016/j.rvsc.2024.105500
PMID: 39647213

3 p, 747.2 KB

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