Fecal metabolomics to understand intestinal dysfunction in male dairy beef calves at arrival to the rearing farm
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Amigó, Núria (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Pérez-Rodado, Marc (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Saco Rodriguez, Yolanda (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Peña, Raquel (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Pato, Raquel (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Pisoni, Lucía (Institut de Recerca i Tecnologia Agroalimentàries)
Devant Guille, Maria (Institut de Recerca i Tecnologia Agroalimentàries)
Marti, Sonia (Institut de Recerca i Tecnologia Agroalimentàries)

Date:	2025
Abstract:	Fecal biomarkers are becoming an important analytical tool since feces are in direct contact with the inflamed intestine and site for the gut microbiome. The objective of this study was the identification of differential fecal metabolites by means of 1 H-NMR to evaluate the management of male dairy beef calves, and which could become potential biomarkers of gastrointestinal disorders. Holstein calves were subjected to a protocol aimed to simulate real conditions of the dairy beef market. Three groups were studied: Control (CTR: high colostrum, no transport, milk replacer), LCMR (low colostrum, transport, milk replacer) and LCRS (low colostrum, transport, rehydrating solution). Fecal lactoferrin was determined as marker of intestinal inflammation, and metabolomic profiling was performed in feces collected the day after arrival to the farm. 41 polar and 10 non-polar metabolites were identified, of which proline, formate and creatine increased in the LCRS group, whereas butyrate and uracil decreased. Less differences were found in non-polar metabolites. Multivariate analysis indicated that most differences are found between the LCRS group and the others. In conclusion, this study indicates that feed restriction has a more important effect at this age than colostrum uptake and transport. These results should help to identify robust fecal biomarkers to assess calf intestinal health and improve management protocols.
Grants:	Agencia Estatal de Investigación PID2019-104021RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00642
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	1 H-NMR ; Dairy beef calves ; Feces ; Inflammation ; Lactoferrin ; Metabolomics ; Biochemistry ; Biological techniques ; Biomarkers
Published in:	Scientific reports, Vol. 15 (february 2025) , ISSN 2045-2322

DOI: 10.1038/s41598-025-90407-3
PMID: 40011507

15 p, 3.2 MB

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