Phenotypical, Behavioral, and Systemic Hallmarks in End-Point Mouse Scenarios
Castillo-Mariqueo, Lidia (Universidad Católica de Temuco)
Alveal-Mellado, Daniel (Universidad Católica de Temuco)
Gimenez-Llort, Lydia (Universitat Autònoma de Barcelona. Departament de Psiquiatria i de Medicina Legal)

Fecha:	2025
Resumen:	The state of frailty is a clinical-biological syndrome that affects the older population with a higher risk of functional dependence. Animal models can provide a tool to study this complex scenario. In the present work, we analyzed the physical and behavioral hallmarks of end-point status in 16-month-old mice (C57BL/6J) according to animal welfare regulations compared to age-matched counterparts with normal aging. A group of 6-month-old mice was added to control for age bias. First, we identified 'structural kyphosis' (visible and unmodifiable deformation in locomotion) correlated with piloerection as the hallmarks of the physical frailty phenotype compared to the 'postural kyphosis' (adjustment to counteract increased visceral volume but attenuated during locomotion) of old mice with normal aging. Alopecia (barbering) was presented in both old groups. Normal levels of exploratory activity in the corner test for neophobia and triceps surae muscle weight but an increased latency of rearing indicated the poorest emotional phenotype, with a possible contribution of structural kyphosis. The presence of hepatomegaly and splenomegaly counteracted the significant WAT loss commonly associated with end-of-life traits, which should have a normal body weight but preserved muscle mass.
Ayudas:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-01468 European Commission 737390
Nota:	Altres ajuts: Memorial Mercedes Llort Sender 2021/80/09241941.8.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	End-point ; Animal welfare ; Euthanasia ; Bodyweight ; Sarcopenia ; C57BL/6J mice
Publicado en:	Animals, Vol. 15, Núm. 4 (February 2025) , ISSN 2076-2615

DOI: 10.3390/ani15040521
PMID: 40003003

15 p, 1.7 MB

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