Whole-genome sequencing of Sphingobium baderi SC-1 and identification of a crucial 3-phenoxybenzoic acid-degrading gene
Li, Qin (Sichuan Agricultural University)
Zhou, Qiao (Sichuan Agricultural University)
Chen, Yuan (Sichuan Agricultural University)
Hu, Kaidi (Sichuan Agricultural University)
Sarrà i Adroguer, Montserrat (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Li, Jianlong (Sichuan Agricultural University)
Liu, Aiping (Sichuan Agricultural University)
Zou, Likou (Sichuan Agricultural University)
Liu, Shuliang (Sichuan Agricultural University)

Fecha:	2024
Resumen:	As an efficient degradation strain, Sphingobium baderi SC-1 can breakdown 3-phenoxybenzoic acid (3-PBA) with high proficiency. To investigate the internal factors that regulate this process, we conducted whole-genome sequencing and successfully identified the pivotal 3-PBA-degrading gene sca (1,230 bp). After sca was expressed in engineered bacteria, a remarkable degradation efficiency was observed, as 20 mg/L 3-PBA was almost completely decomposed within 24 h. The phenol was formed as one of the degradation products. Notably, in addition to their ability to degrade 3-PBA, the resting cells proficiently degraded 4'-HO-3-PBA and 3'-HO-4-PBA. In conclusion, we successfully identified and validated sca as the pivotal enzyme responsible for the efficient degradation of 3-PBA from Sphingomonas baderi, providing a crucial theoretical foundation for further explorations on the degradation potential of SC-1.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Sphingobium baderi SC-1 ; Whole-genome sequencing ; 3-phenoxybenzoic acid-degrading gene ; Resting cells ; Degradation characteristics
Publicado en:	Frontiers in microbiology, Vol. 15 (April 2024) , art. 1361335, ISSN 1664-302X

DOI: 10.3389/fmicb.2024.1361335
PMID: 38646623

12 p, 2.5 MB

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