DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers
Soukarie, Diana (Basque Research & Technology Alliance. CIC nanoGUNE)
Nocete, Lluis (Universitat Autònoma de Barcelona)
Bittner, Alexander M. (IKERBASQUE Basque Foundation for Science)
Santiago, Ibon (Basque Research & Technology Alliance. CIC nanoGUNE)

Fecha:	2024
Resumen:	Incorporating biomolecules as integral parts of computational systems represents a frontier challenge in bio- and nanotechnology. Using DNA to store digital data is an attractive alternative to conventional information technologies due to its high information density and long lifetime. However, developing an adequate DNA storage medium remains a significant challenge in permitting the safe archiving and retrieval of oligonucleotides. This work introduces composite nucleic acid-polymer fibers as matrix materials for digital information-bearing oligonucleotides. We devised a complete workflow for the stable storage of DNA in PEO, PVA, and PCL fibers by employing electrohydrodynamic processes to produce electrospun nanofibers with embedded oligonucleotides. The on-demand retrieval of messages is afforded by non-hazardous chemical treatment and subsequent PCR amplification and DNA sequencing. Finally, we develop a platform for melt-electrowriting of polymer-DNA composites to produce microfiber meshes of programmable patterns and geometries.
Ayudas:	Ministerio de Ciencia e Innovación CEX2020-001038-M Agencia Estatal de Investigación PID2019-104650GB-C22 European Commission 101115292
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	DNA data storage ; Nanofibers ; Melt-electrowriting ; Electrospinning ; Additive manufacturing
Publicado en:	Materials Today Bio, Vol. 24 (February 2024) , art. 100900, ISSN 2590-0064

DOI: 10.1016/j.mtbio.2023.100900
PMID: 38234463

9 p, 7.1 MB

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