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| Home > Articles > Published articles > Deciphering the causes of ibfa-mediated abortive infection in the P22-like phage UAB_Phi20 |
(Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia) (Universitat Autònoma de Barcelona. Departament d'Enginyeria de la Informació i de les Comunicacions) (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)| Date: | 2025 |
| Abstract: | The study of bacterial defense mechanisms against phages is becoming increasingly relevant due to their impact on the effectiveness of phage therapy. Employing a multifaceted approach that combines bioinformatics, molecular microbiology, TEM microscopy, and conventional microbiology techniques, here, we identify the ibfA gene as a novel defense factor targeting the virulent phage UAB_Phi20, acquired by Salmonella Typhimurium through lateral transfer on the IncI1α conjugative plasmid pUA1135 after oral phage therapy in broilers. IbfA, a two-domain protein containing ATPase and TOPRIM domains, significantly reduces UAB_Phi20 productivity, as indicated by decreased EOP, ECOI, and a diminished burst size, potentially reducing cellular viability without causing observable lysis. Our results indicate that IbfA enhances the transcription of early genes, including the antirepressor ant, which inhibits the C2 repressor of the lytic cycle. This may cause an imbalance in Cro/C2 concentration, leading to the observed reduction in the transcription of late genes encoding structural and cellular lysis proteins, and resulting in the abortion of UAB_Phi20 infection. |
| Grants: | Agencia Estatal de Investigación PID2023-152240OB-C2 Agencia Estatal de Investigación PID2020-117708GB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00646 Ministerio de Educación FPU19/01182 |
| Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Language: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Subject: | Salmonella ; Phage therapy ; Bacterial defense mechanism ; IncI1α conjugative ; Plasmid ; Ibfa ; Abortive infection |
| Published in: | International journal of molecular sciences, Vol. 26, Issue 10 (May 2025) , art. 4918, ISSN 1422-0067 |
