Efficient sorghum and maize transformation using a ternary vector system combined with morphogenic regulators
Fontanet-Manzaneque, Juan B. (Centre de Recerca en Agrigenòmica)
Haeghebaert, Jari (Ghent University. Department of Plant Biotechnology and Bioinformatics)
Aesaert, Stijn (Ghent University. Department of Plant Biotechnology and Bioinformatics)
Coussens, Griet (Ghent University. Department of Plant Biotechnology and Bioinformatics)
Pauwels, Laurens (Ghent University. Department of Plant Biotechnology and Bioinformatics)
Caño Delgado, Ana I. (Centre de Recerca en Agrigenòmica)

Fecha:	2024
Resumen:	Sorghum bicolor (sorghum) is a vital C4 monocotyledon crop cultivated in arid regions worldwide, valued for its significance in both human and animal nutrition. Despite its agricultural prominence, sorghum research has been hindered by low transformation frequency. In this study, we examined sorghum transformation using the pVS1-VIR2 ternary vector system for Agrobacterium, combined with the morphogenic genes BABY BOOM and WUSCHEL2 and selection using G418. We optimized Agrobacterium-mediated infection, targeting key parameters such as bacterial optical density, co-cultivation time, and temperature. Additionally, an excision-based transformation system enabled us to generate transgenic plants free of morphogenic regulators. The method yielded remarkable transformation frequencies, reaching up to 164. 8% based on total isolated plantlets. The same combination of ternary vector, morphogenic genes and geneticin-based selection also resulted in a marked increase in transformation efficiency of the Zea mays (maize) inbred line B104. The potential for genomic editing using this approach positions it as a valuable tool for the development of sorghum and maize varieties that comply with evolving European regulations. Our work marks a significant stride in sorghum biotechnology and holds promise for addressing global food security challenges in a changing climate.
Ayudas:	European Commission 683163 Agencia Estatal de Investigación PID2020-118218RB-I00
Nota:	Altres ajuts: EMBO Scientific Exchange Grant (number 9889)
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Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Sorghum bicolor ; Transformation ; Morphogenic regulators ; Ternary vector ; Baby Boom ; Wuschel2 ; Zea mays
Publicado en:	The Plant journal, Vol. 120, Issue 5 (December 2024) , p. 2076-2088, ISSN 1365-313X

DOI: 10.1111/tpj.17101

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