Evaluating Host Defense Peptides : A Comparative Analysis of Synthetic Peptides and Recombinant Concatemers
Saubi, Cristina (Institut de Recerca i Tecnologia Agroalimentàries)
Carratalá, José Vicente (Institut de Recerca i Tecnologia Agroalimentàries)
Bello-Madruga, Roberto (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
López-Cano, Adrià (Institut de Recerca i Tecnologia Agroalimentàries)
Navarro, Susanna (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Arís i Giralt, Anna (Institut de Recerca i Tecnologia Agroalimentàries)
Garcia-Fruitos, Elena (Institut de Recerca i Tecnologia Agroalimentàries)

Date:	2025
Abstract:	The global antibiotic resistance crisis raises concerns about antibiotic use, and alternative strategies are urgently needed. In this context, host defense peptides (HDPs) have rapidly gained interest. However, one of the main obstacles is their production strategy. Chemical synthesis is the most widely used, although it is not scalable and has sequence limitations. A possible alternative is recombinant production, but the strategies used so far have limited efficiency. In this study, we aim to compare the activity and main characteristics of different HDPs produced by both chemical synthesis and by recombinant production, using an approach based on tetramers to ameliorate the production process. The results obtained showed that the production of HDPs as tetrameric peptides by recombinant production in Lactococcus lactis enhanced the peptide activity, with HDPs being much more active in terms of antimicrobial activity, more structurally stable, and nanostructured. Thus, the recombinant strategy described herein, fusing four repetitions of the same peptide, can become a real alternative to produce highly active HDPs through a scalable production process.
Grants:	Agencia Estatal de Investigación PID2022-136521OB-I00 Ministerio de Ciencia e Innovación PRE2021-097678 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-01552
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Host defense peptides ; Recombinant production ; Concatemers ; Antimicrobial activity ; Lactococcus lactis
Published in:	Biomolecules, Vol. 15, Issue 7 (July 2025) , art. 980, ISSN 2218-273X

DOI: 10.3390/biom15070980
PMID: 40723852

20 p, 3.4 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles