Application of Combined Irradiation Mutagenesis Technique for Hyperproduction of Surfactin in Bacillus velezensis Strain AF_3B
Farooq, Syeda Amna (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Khaliq, Shazia (Pakistan Institute of Engineering and Applied Sciences)
Ahmad, Saeed (Pakistan Institute of Engineering and Applied Sciences)
Ashraf, Neelma (Albert-Ludwig University of Freiburg)
Ghauri, Muhammad Afzal (Pakistan Institute of Engineering and Applied Sciences)
Anwar, Munir Ahmad (Pakistan Institute of Engineering and Applied Sciences)
Akhtar, Kalsoom (Pakistan Institute of Engineering and Applied Sciences)

Date:	2025
Abstract:	The major challenge in large-scale industrial use of lipopeptide surfactin is the low yield by indigenous bacterial strains and the higher cost of its production that have been proved as a limiting factor in commercial applications. Therefore, there is an urgent demand for high-yielding strains that can be achieved through strain improvement. A first report on the use of a combination of UV and gamma-irradiation mutagenesis for the development of surfactin hyperproducing mutants of Bacillus spp. proved to be significant and resulted in a twofold enhancement in surfactin yield. The mutant was able to grow and produce surfactin on all the tested carbon and nitrogen sources, while 2% glycerol favored maximum surfactin yield (1. 62 g/L) as compared to the wild-type strain that showed a maximum 0. 85 g/L surfactin yield at 3% sucrose. Additionally, the mutant exhibited a good yield of pure surfactin, that is, 1. 55 g/L as compared to the wild strain (0. 411 g/L) by using corn steep liquor as the main component of the fermentation medium. The study concluded overall a threefold enhancement in the relative abundance of purified surfactin and its isoforms detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis in mutant strain AF-UVγ2500.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	International Journal of Microbiology, Vol. 2025 (february 2025) , ISSN 1687-9198

DOI: 10.1155/ijm/5570585
PMID: 40018418

16 p, 2.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Engineering > Composting Research Group (GICOM)
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