Utilization of discard bovine bone as a support for immobilization of recombinant Rhizopus oryzae lipase expressed in Pichia pastoris
Clementz, Adriana L. (Universidad Nacional del Litorial. Instituto de Investigaciones en Catálisis y Petroquímica (Argentina))
Del Peso, Gonzalo (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Canet, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Yori, Juan C. (Universidad Nacional del Litorial. Instituto de Investigaciones en Catálisis y Petroquímica (Argentina))
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Fecha:	2016
Resumen:	In this study the possibility of using discard bovine bone as support for immobilization of Rhizopus oryzae lipase expressed in Pichia pastoris was analyzed. Discard bovine bone were milled and then subjected to a chemical treatment with acetone in order to remove lipids and blood traces. Two types of supports were evaluated: bovine bone and calcined bovine bone for 2 h at 600°C. Supports were characterized by: ICP, SEM, XRD, FTIR, XPS, and N2 adsorption isotherms. Calcined bovine bone presented appropriate characteristics for the lipase immobilization due to the removal of collagen: high porosity, large surface area and suitable porous structure. Biocatalysts were prepared with different initial enzyme load. For the equilibrium adsorption studies, the Langmuir isotherm was used to fit the data results. The immobilization occurs in monolayer to a value of 35 UA mg-1. The activities of biocatalysts were tested in transesterification reaction of olive oil. For the enzyme load used in the test, a final yield percentage of 49. 6 was achieved after six methanol additions and 180 min of reaction, similar values were obtained using Relizyme as support. Therefore, the bovine bone discard is an economical and appropriate choice for use support immobilization of enzymes.
Ayudas:	Ministerio de Economía y Competitividad CTQ2013-42391 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-452
Derechos:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Biocatalysis ; Biodiesel ; Bovine bone ; Lipase immobilization ; Transesterification
Publicado en:	Biotechnology Progress, Vol. 32, Issue 5 (September-October 2016) , p. 1246-1253, ISSN 1520-6033

DOI: 10.1002/btpr.2321

Postprint 28 p, 2.0 MB

El registro aparece en las colecciones:
Artículos > Artículos de investigación
Artículos > Artículos publicados