Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase
Duarte, Susan Hartwig (Universidade de Campinas. Departamento de Engenharia de Alimentos)
Peso Hernández, Gonzalo Lázaro del (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Canet Morral, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Maugeri, Francisco (Universidade Estadual de Campinas)
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)

Date:	2015
Abstract:	The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40. 6% was obtained with microbial oils lower than olive oil 54. 3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.
Grants:	Ministerio de Economía y Competitividad CTQ2013-42391-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-452
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Biodiesel ; Rhizopus oryzae lipase ; Single cell oil
Published in:	Bioresource Technology, Vol. 183 (May 2015) , p. 175-180, ISSN 1873-2976

DOI: 10.1016/j.biortech.2015.01.133

Postprint 27 p, 399.2 KB

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